辛伐他汀诱导K562细胞凋亡过程中.pdf

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肿瘤防治研究 2007 年第 34 卷第 1 期· 93 ·
辛伐他汀诱导 K562 细胞凋亡过程中 2 2
Caspase 3 、Caspase 9 活性变化
黄文芳1 ,杨永长2 2 2,刘华1 ,陈 2江22 ,周定安2
Changes of Caspase 3 and Caspase 9 Activity during the Simvastatin induced Apoptosis in
K562 Cells 2
HUAN G Wen fang1 , YAN G Yong chang2 ,L IU Hua1 ,CH EN Jiang2 ,ZHOU Ding an2
1. Department of Clinical L aboratory , S ichuan Medical Science A cadem y S ichuan Provincial People’s
Hos pital , Cheng du 610072 , China; 2. Department of L aboratory Medicine , Chongqing University of
Medical Sciences
Abstract :Objective To observe the variations of Caspase 3 and Caspase 9 activities in the process of
simvastatin induced apoptosis in K562 cells. Methods K562 cells were exposed to different concentra
tions of simvastatin ,cell morphological analysis and flow cytometry were performed to confirm cell apop
tosis. Colorimetric method was used to measure Caspase 3 and Caspase 9 activities. Results K562 cells
could be induced to undergo apoptosis after 5 ,10 ,20μmol/ L simvastatin treatment for 48h ,and the apop
totic rate was (4. 00 ± 0. 13) % , (6. 24 ± 0. 18) % and (9. 41 ± 0. 22) % respectively ,compared with the
control group ,it was significantly higher ( P < 0. 01) . At 72h , K562 cells were apoptotic with a rate of
(7. 62 ± 0. 21) % , (12. 41