低氧对间充质干细胞的影响论文.doc

低氧对间充质干细胞的影响论文.doc低氧对间充质干细胞的影响论文
【摘要】氧对于几乎所有的生命都是必需的,它维持能量代谢及细胞的功能,但机体多种细胞是处于生理或病理性低氧状态中,而体外细胞实验一般是在20%氧浓度下进行,并不符合生理状态。直到最近,关于低氧对于间充质干细胞(mesenchymal stem cells, MSC)的影响有了一系列的研究。体外实验表明,适度低氧下MSC的生长和存活能力更好,氧浓度可以影响MSC向成骨、成软骨、 Stem Cells ——Revieed under 20%O2 , that do not replicate normal physiological hypoxia or pathological hypoxia conditions in the body. Recently, the effect of hypoxia on mesenchymal stem cells (MSCs) has been studied, under physiological hypoxia, MSCs thrive igration are changed. Hypoxia changes the physiological characteristics of embryonic stem cell, hematopoietic stem cell and neuron stem cell as echanism of these responses might be primarily involved in the hypoxic inducible factor1 (HIF1) signal pathphasizes that hypoxia is an important factor on all major aspects of stem cell biology including survival, proliferation, differentiation, and migration, and the mechanism involved in HIF1 signaling pathesenchymal stem cell; cell proliferation; cell differentiation; cell migration
间充质干细胞(mesenchymal stem cells,MSC)具有强大的自我更新及多系分化潜能,体内移植后可以迁移至损伤的部位(多数为缺血缺氧的环境)修复相应的组织。干细胞体外增殖分化的研究通常是在20%O2的条件下,然而体内氧浓度显著低于此值,动脉血氧浓度约为12%,组织的平均氧浓度约为3%,.freelar等[3]发现,% O2(极度低氧)下兔来源的干细胞死亡增多,但他们同时证明在有限的氧浓度下干细胞依旧可以存活。 Greijer等[4]的实验表明,缺氧的严重程度决定着细胞凋亡或者适应缺氧而存活, % O2可以启动细胞的凋亡,从而阻止低氧诱导突变的积累,在此过程中存在着复杂诱导凋亡和抗凋亡间的平衡,涉及低氧诱导因子1(hypoxic inducible foctor1, HIF1)与多种因子的结合,% O2的