Chapter2DNAStructureandproperties§2-icMaterial§2-2TheChemicalNatureofPolynucleotides§2-3DNAisaDoubleHelix§2-4PolymorphismofDNAStructure§2-5DenaturationandRenaturation§2-6SupercoiledDNAandTopoisomerase§2-7RestrictionEnzymesandDNASequencing§2-icmaterialdeoxyribonucleicacid(DNA)DNAisthestorehouse,orcelllibrary,(1869)pounds—,—fromvirulent(S)smoothcolonieswherebacterialhadcapsules,toavirulent(R)roughcolonieswithoutcapsulesHeat-killedvirulentcoloniescouldtransformavirulentcoloniestovirulentonesDNA:TheTransformingMaterialIn1944AveryusedatransformationtestsimilartoGriffith’,1928;Averyetal.,,esfromDNABy1953Watson&Crickpublishedthedouble-helicalmodelofDNAstructureandChargaffhadshownthatthe4baseswerenotpresentinequalproportions1952MarthaChase&?icmaterial?复制?表面遗传信息?羊痒疫(scripie)人类kuru病bovingspongiformencephalopathy(疯牛病)高度纯化的病体脑组织的感染性粒子可被蛋白酶K抑制不被核酸酶和辐射灭活不含大于100nt的核酸28kD的疏水性糖蛋白由核基因组基因PrP(PrionrelatedProtein)编码Prion(ousinfectionsparticle)传染性病原蛋白颗粒引起的风波
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