铜尾矿废弃地生物土壤结皮固氮微生物多样性_詹婧.pdf

1铜尾矿废弃地生物土壤结皮固氮微生物多样性*詹婧阳贵德孙庆业**(安徽大学资源与环境工程学院,合肥 230601)摘要生物土壤结皮能够有效提高矿业废弃地有机质和氮的积累,,以铜陵铜尾矿废弃地3种类型的生物土壤结皮(藻结皮、藓结皮和藻-藓混合结皮)为研究对象,利用变性梯度凝胶电泳(DGGE),其次为维管植物群落下的藻-藓混合结皮,苔藓结皮具有最低的固氮蓝藻多样性;随着维管植物群落高度和盖度的增加,固氮微生物多样性降低,铜尾矿废弃地的pH、水分、有机质、养分含量(氮和磷),废弃地结皮中固氮微生物以蓝藻为主, PCR- of nitrogen-fixing anisms in biological soil crusts of copper mine wastelands. ZHAN Jing, YANG Gui-de, SUN Qing-ye(School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China)Abstract: Biological soil crusts play an important role in anic matter and nitrogen in the revegetationof mining wastelands. The diversity of nitrogen-fixing anisms in three types of biological soil crusts (algal crust, moss crust and algal-moss crust) from two wastelands of copper mine tailings were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis, based on the nifH gene of diazotrophs, to investigate: 1) the diversity of nifH gene in the crusts of mine wastelands, and 2) whether and how the nifH gene diversity in the crusts could be affected by the development of munities. The algal crust on the barren area displayed the highest nifH gene diversity, followed by the algal-moss crusts within vascular munities, and the moss crust displayed the lowestnifH gene diversity. The diversity of diazotrophs in algal-moss crust within vascular plant communities decreased with the increase of height and cover of vascular plant communities. No significant relationship was found between wasteland properties (pH, water content, contents of organic matter, nitrogen and phosphorus and heavy metal concentration) and nifH gene diversity in the crusts. Sequencing and ic analysis indicated that most nitrogen-fixing