建立高氯酸比色法测定黄药子总皂苷含量.doc

Introduction
Rhizoma Dioscoreae bulbiferae is the tuber of Dioscorea bulbifera L. in Dioscoreaceae. Its drug property y administering the Dioscorea bulbifera and mouse livers lost the ability to detoxify since the livers were severely damaged by cumulative poison in the late of administration. There were(are)two ways that led(leading )to liver damage. One was(is) a direct toxic effect on liver cells. The other (one)was (is )that a indirect toxic effect which inhibited the activity of antioxidant enzymes and drug metabolism in liver microsomes by inducing the expression of mRNA of CYP1A2 and CYP2E1 to translate early poisons into substances of hepatotoxicity. The hepatocytes of mouse had cancerous trends in the(at a )later stage of Dioscorea bulbifera poisoning. Fas-associated proteins were involved in the process of cell apoptosis. The gene (BC065098) was up-regulation.
Dioscorea Bulbifera contains a variety of chemical composition, such as steroidal saponins，diterpene lactone，emodin，flavonoids，some trace elements and so on. In recent years, domestic scholars focused their studies on the second terpene esters of Disocorea Bulbifera(Dioscorea Bulbifera)causing liver damage. The Dioscorea bulbifera saponins have been widely thought to cause hepatotoxicity. However, there have been few studies and reports about(on)the Dioscorea bulbifera saponins，the content analysis
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