小肠粘膜下层材料复合体修复大块关节软骨缺损的实验研究.pdf

华中科技大学硕士学位论文小肠粘膜下层材料复合体修复大块关节软骨缺损的实验研究姓名:雷中华申请学位级别:硕士专业:外科学(骨科)指导教师:王洪20090401华中科技大学硕士学位论文1 小肠粘膜下层材料复合体修复大块关节软骨缺损的实验研究硕士研究生雷中华导师王洪副教授研究目的:观察小肠粘膜下层材料与β-磷酸三钙复合后结合自体骨髓基质干细胞修复大块关节软骨缺损的治疗效果;观察添加转化生长因子-β1对软骨缺损的修复的治疗效果;分析探讨软骨缺损修复的具体机制,为进一步研究和临床应用提供实验依据。研究方法:小肠粘膜下层材料与β-磷酸三钙复合材料用SIS膜包绕,作为支架材料,穿通骨髓腔利用自体骨髓基质干细胞作为种子细胞,用来修复大块关节软骨缺损。对复合材料进行电镜扫描。选取新西兰大白兔36只随机分成A组(关节软骨全层缺损),B组(SIS材料复合体植入)和C组(SIS材料复合体添加转化生长因子β1植入)。植入术后12周进行大体观察、标本的组织学观察和免疫组化染色、改良Wakitani score法评价软骨修复并进行统计学分析。研究结果:复合材料电镜扫描呈疏松多孔状。B、C组HE染色均可见大量类软骨细胞出现,SIS材料成分基本降解,β-磷酸三钙粉末少量残留,周围无明显淋巴细胞和浆细胞浸润;B、C组II型胶原免疫组化染色阳性。半定量的改良Wakitani score法统计结果显示B组、C组与A组各单项比较普遍存在显著性差异(p<);B组和C组各单项比较无显著性差异(p>),但是总分比较存在显著性差异(p<)。研究结论:小肠粘膜下层与β-磷酸三钙等比例复合材料是一种优良的软骨组织工程用支架材料;转化生长因子β1对软骨组织形成中有积极的促进作用。关键词:小肠粘膜下层;β-磷酸三钙;骨髓间充质干细胞;支架材料;关节软骨;组织工程华中科技大学硕士学位论文2 The Experimental Study of Repairing Rabbit Tibial Cartilage Defect by plex Medical master Lei Zhonghua Tutor Prof. Wang Hong Abstract Objectives:To observe the effect of large articular cartilage defect repaired with autologous mesenchymal stem cells and scaffold material mixed with small intestinal submucosa and β-tricalcium phosphate powder,observe the effect of TGF-β1 on cartilage tissue engneering,and to provide experimental basis for clinical applications. Methods:In this study,large articular cartilage defect were repaired with two parts :scaffold material mixed with small intestinal submucosa and β-tricalcium phosphate powder,and seed cells from autologous mesenchymal stem cells by rating the bone marrow cavity. scaffold material were studied by scanning electron New Zealand White rabbits were randomly devided into 3 groups: group A(articular cartilage defect were made), group B(plex were transplanted), group C(plex that contain TGF-β1 were transplanted). Twelve weeks later,general observation,histological test,Wakitani score and immunohistochemical examination were observed,and then,statistical analysis of Wakitani score data was made. Results:The scannin