国外大学细胞生物学课件 英文课件12.ppt

D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. 1 Lecture 12: Vesicle formation and fusion D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. 2 Protein sorting in the cytosol - summary D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. 3 D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. Vesicular transport 4 Questions to consider: How is cargo selected? How is cargo separated from resident proteins of the anelle? How do vesicles fuse? How is a vesicle targeted to fuse with a anelle? D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. 5 How are vesicles formed? The recruitment of a small GTPase to the donor membrane is the first step of vesicle formation. The main function of the coat is the selective recruitment of cargo into the budding vesicle. Coat and cargo recruitment vesicle buds off D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. 6 The mechanism of vesicle formation is conserved Lee et al., 2004 D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. 7 Vesicles look similar by electron microscopy D103 W2011 Cell Biology - lecture 12- copyright ? Suetterlin and MacGregor - all rights reserved. A link between ponents and disease 8 ? General defects in protein secretion ? cell death ? Protein secretion is an essential process ? Mutations that only block the secretion of specific proteins ? Sec23A: linked to a rare cranofacial disorder that causes skeletal disorders ? Sec24B: defects in neural tube closure because a receptor required for the development of the neural epithelium is not trafficked properly ( Merte et al., 2010) D103 W2011 Cell Biology - lecture