Amoeba and flagella 阿米巴和鞭毛虫. Entamoeba histolytica (E. histolytica ) 溶组织内阿米巴. Giardia lamblia (G. lamblia )兰氏贾第鞭毛虫. Trichomonas vaginalis (T. vaginalis )***毛滴毛虫. Leishmania donovani (L. donovani )杜氏利什曼原虫 Purpose and Requirements .Master morphological features of trophozoite and cyst of Entamoeba histolytica and Giardia lamblia ; Master movement characteristics of trophozoites ; .Master morphological features and movement characteristics of Trichomonas vaginalis ; .Familiar with differtiations of E. histolytica cyst and other non-pathogenic amoebae cyst; .Familiar with iodine stain for cyst; .Understanding Balantidium coli ; .Understanding morphological features of amastigote of Leishmania donovani and sandfly . . No. 原-1至 No. 原-4: : 1)图片及视频示教: 2)镜下示教标本: : ①直接涂片法观察活动的阿米巴滋养体; ②直接涂片法观察活动的***毛滴虫; ③碘染色法观察包囊 : 本次课学****任务 of glass slide No. 原-1至 No. 原-4 拿到玻片标本后请马上检查核对是否有漏、重复或破损,有问题的请马上举手报告老师! No. 原-1: 溶组织内阿米巴包囊、结肠内阿米巴包囊、微小内蜓阿米巴包囊、布氏嗜碘阿米巴包囊 Cyst of E. histolytica , E. coli, E. nana, I. buetschlii No. 原-2: 蓝氏贾第鞭毛虫滋养体 G. lamblia trophozoite (仅双号同学有, 2个同学共用 1张) No. 原-3: 蓝氏贾第鞭毛虫包囊 G. lamblia cyst No. 原-4: ***毛滴虫滋养体 T. vaginalis trophozoite 注意:①在观察玻片标本前,先将显微镜光线调到最强! ②本次课只使用 10倍镜和油镜; No. 原-4:***毛滴虫滋养体 T. vaginalis trophozoite Axostyle 轴柱 4 anterior flagellum 4 根前鞭毛 Lateral flagellum and Undulating membrane 波动膜 Nucleus of glass slide No. 原-1至 No. 原-4 先10倍镜下调出焦距平面,然后转油镜下观察细微结构要求拍照姬姆萨染色法( Giemsa staining method ) No. 原-3:蓝氏贾第鞭毛虫包囊 G. lamblia cyst Steined with iron- hematoxylin Cyst wall 囊壁 Nucleus 核 Axostyle 轴柱 Median body 中体. Cyst wall : . Axostyle : . Median body : . Nucleus : of glass slide No. 原-1至 No. 原-4 先10倍镜下调出焦距平面,然后转油镜下观察细微结构要求拍照 No. 原-2:蓝氏贾第鞭毛虫滋养体 G. lamblia trophozoite Anterior flagellum 前鞭毛 Ventral flagellum 腹鞭毛 Posterior flagellum 后鞭毛 Caudal flagellum 尾鞭毛 Adhesive disc 吸盘 Nucleus
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