抗柯胶囊总皂苷对柯萨奇病毒B型诱导细胞凋亡的抑制作用 张之斌.pdf

中国药物警戒 第 19 卷第 4 期 2022 年 4 月 April, 2022, ,
DOI:- 中图分类号： China University of Sciences and Technology, Tangshan Hebei 063210, China; Institute of Traditional
4
Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Institute of Chinese
Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China)
Abstract: Objective
To observe the inhibitory effects of saponins from Kangke capsules on apoptosis caused by
Methods
Coxsackievirus B (CVB), and to explore the mechanism. CVB was inoculated into cardiomyocytes to
establish an infection model in vitro. The cytopathic effect method (CPE) was adopted to determine the 50% inhibitory
concentration (IC50) and therapeutic index (TI) of saponins from Kangke capsules on CVB3, CVB4 and CVB5. CCK-8 kit
was used to detect cell viability. TdT-mediated dUTP notch end labeling technique (TUNEL) was used to determine the
apoptosis rate of cardiomyocytes. Western-blot was used to study the expressions of Bcl-2 and Bax protein in primary
Results
rat cardiomyocytes. Saponins from Kangke capsules could significantly