伸筋草超临界萃取物工艺参数优化及药效验证.doc

1 伸筋草超临界萃取物工艺参数优化及药效验证【摘要】目的考察超临界 CO2 流体萃取伸筋草有效成分的最佳工艺, 进一步验证萃取物的药效。方法采用正交实验设计,以萃取物得率为指标,考察萃取压力、萃取温度、解析压力及解析温度 4 个因素的影响; 采用热板法、醋酸扭体法、鼠耳二甲苯致炎法、棉球肉芽肿致炎法观察萃取物的镇痛抗炎效果。结果最佳萃取工艺条件为萃取压力 20 mPa ,萃取温度 35℃,解析压力 8 mPa ,解析温度 55℃,萃取时间 4h ;萃取物对热致痛和冰乙酸所致的小鼠扭体疼痛具有良好的镇痛作用, 对二甲苯致小鼠耳炎有显著的消炎作用。结论超临界 CO2 流体萃取技术用于伸筋草有效成分提取, 方法简单, 适用于工业生产。伸筋草超临界萃取物具有显著镇痛抗炎的药理作用。【关键词】伸筋草; 超临界二氧化碳萃取; 抗炎; 镇痛 Optimization on Craft and Drug Action Validation of SFE 2 拟 CO2 Extract for Lycopodium japonicum Thunb. Abstract:ObjectiveTo study the optimal technology of supercritical 拟 CO2 fluid extraction(SFE 拟 CO2) on Lycopodium japonicum validate the drug action of The yield of extractant was used as index to evaluate the technologies based on orthogonal design , in which 4 factors considered were extraction temperature and pressure , separation temperature and pressure;the anti- inflammation and analgesia pharmacological actions was observed by drug action optimum extraction conditions were : extraction pressure 20MPa, temperature 35℃;separation pressure 8MPa, temperature 55℃; extraction time 4 extract had good analgesia pharmacological action to pain caused by heat in mice and by acetic acid causing body distortion test in mice. It also had significant depressions to the inflammation caused by dimethyl benzene on the ear of fluids extrac