养阴抗毒胶囊2号对阴虚大鼠糖皮质激素受体蛋白表达的影响.doc

1 养阴抗毒胶囊2 号对阴虚大鼠糖皮质激素受体蛋白表达的影响作者:丁鑫,侯颖,雎岩, 史恒军【关键词】养阴抗毒胶囊 2 号;糖皮质激素类;阴虚;糖皮质激素受体;大鼠【 Abstract 】 AIM: To observe the effect of Nourishing Yin Antitoxic Capsule 2 (YC2) on the expression of glucocorticoid receptor protEins (GR) in Yin deficiency rats induced by hydrocortisone, and to investigate how the YC2 relieves the side effects of Glucocorticoid. METHODS: Thirtytwo SD rats were randomly allocated to4 groups: normal control group, Yin deficiency model control (YD) group, YC2 group and Guifu Dihuang Balls (GFB) group. Radioimmunological method was used to observe the contents of corticosteroid (CORT). Immunohistochemistry was used to observe the expression of GR in the liver cell nucleus. RESULTS: Compared with the normal group, the contents of CORT were higher in YD group (), which was ( ± )μ g/L, and the expression 2 of GR in the liver cell nucleus was lower for the optical density ratio of immunohistochemistry stain ( ± , ). Compared with the YD group, the contents of CORT in YC2 group were lower ( ), which was ( ± ) μ g/L, and the expression of GR in the liver cell nucleus was higher for the optical density ratio of immunohistochemistry stain ( ± , ); the contents of CORT in GFB group were little lower ( ), and the expression of GR in the liver cell nucleus was little higher ( ). CONCLUSION: In this experiment, the contents of CORT in YD group were obviously higher than the normal group, and the expression of GR in the liver cell nucleus was lower. The YC2 can increase the expression of GR in the liver cell nucleus, and improve the hyperfunction of hypothalamicpituitaryadrenal (HPA) axis. 【 Keywords 】 Nourishing Yin Antitoxic Capsule 2; glucocorticoids; Yindeficiency; glucocorticoid receptor; rats 【摘要】目的: 观察养阴抗毒胶囊 2号( YC2 ) 对阴虚大鼠模型肝组织糖皮质激素受体( GR ) 蛋白表达的影响和对减轻激素副作用机理. 方法: SD 大鼠 32 只,随机分为空白对照组、阴虚模型组( YD )、 YC2 组和桂附地黄丸组( GFB ). 3 大鼠在中药预处理及造模给药 9d 后处死,用放射免疫方法检测各组血浆皮质醇( CORT )含量,免疫组织化学染色技术测定肝组织细