慢病毒载体介导RNA干扰沉默Livin基因对大肠癌裸鼠移植瘤放射敏感性的影响.pdf

慢病毒载体介导RNA干扰沉默Livin基因对大肠癌裸鼠移植瘤放射敏感性的影响.pdf!!!!!!!!!!!!i!!!!!!Q!!!!!!!!!!!!!!!!!463
放射生物学·
慢病毒载体介导扇懦聊琇基因
对大肠癌裸鼠移植瘤放射敏感性的影响
魏芹何文静陈少卿余娜莎黎军和熊建萍
【摘要】 RNALivinHT-29
鼠移植瘤生长及放射敏感性的影响。方法 36BALBc(nunu)
HT-29
重及瘤体积的变化。—⒚庖咦橹Х治鯨及蛋白表达的变化,原位末端标记法
(TUNEL)10
6 X(5004
%,瘤体重量明显减轻.,,
..%和阴性对照组的
..,。%,明显低
.±%.,。实验
(2367225)(500150)(833
%:鲜保闶笠浦擦鎏寤诜肿橹渥芴宕嬖诓钜
1070P<001)(F=701932P<001)
RNALivinHT-29
并增加移植瘤对放疗的敏感性。
【关键词】慢病毒载体;扇牛籐基因;一细胞;裸鼠移植瘤
Livin RNA HT-29 xenograft
WEI QinHE, —, NashaLI籢琗甌
First , 330006China
Corresponding : JunheEmaill#******@163eom
Abstract0bjective explore the effects silencing Livin RNA
一 xenografi and sensitivity
miceMethods BALBc established inoculating differently
treated 一 cells and the tumor situation was measuring the
and the the difierent after 甃
was
was and ,瓵
Livin shRNA
TUNELNormal, earring unrelated sequenceslentivirusearring
intratumorallyAllthe given 6 甌 changes
and the tumor difierent and the and tumor
甊 The tumor was(5004007)andthe tumor
the
the RNA interfering group was significantly group
the tumor
blank and groups(F=485P<005)and .
%. Livin level the RNA interfering group .±
008)andwas significantly lower the blank group(6760005)andthe
.士% protein level the RNA inferring
group was significantly lower[(3600%.ィ.±%,
was
apoptosis the RNA interfering group significantly