大蒜素抑制人脑胶质瘤U251 细胞生长的实验研究.pdf.pdf

·2745· 中华临床医师杂志(电子版)2012 年 9 月第 6 卷第 18 期( ), 15,2012, .6, .18
Chin J Clinicians Electronic Edition September Vol No
· 论著·
大蒜素抑制人脑胶质瘤 251 细胞生长的
U
实验研究
王宝刘晓东苗旺范益民王宏勤刘祚汝
【摘要】目的探讨大蒜素对体外培养的人脑胶质瘤 251 细胞增殖及诱导凋亡的作用。方法用不
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同浓度大蒜素作用于体外培养的 251 细胞 12 、24 及 48 , 唱8 法检测细胞存活率;流式细胞术( )
U h h K FCM
检测细胞周期及细胞凋亡。结果大蒜素能明显抑制细胞生长,呈量唱效和时唱效关系(P <0畅001); 检测
FCM
表明,细胞主要被阻滞在 0 /1 期;大蒜素诱导细胞凋亡率较对照组明显增加(P <0畅001),有浓度依赖性。
G G
结论大蒜素能抑制 251 细胞增殖,诱导细胞凋亡。
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【关键词】大蒜辣素; 神经胶质瘤; 细胞凋亡
Proliferation inhibition and apoptosis induction of allicin on glioma U251 cells WANG Bao,LIU Xiao唱dong,
MIAO Wang,FAN Yi唱min,WANG Hong唱qin,LIU of Neurosurgery,First Affiliated Hospital,Shanxi
Medical University,Taiyuan 030001,China
Corresponding author:FAN Yi唱min,Email:fanyimin5678@
【Abstract】 Objective
To investigate proliferation inhibition and apoptosis induction of allicin on glioma
251 in 251 in vitro .
U cells U glioma cells cultured were incubated with different doses of allicin The
12,24 48 唱8
cell survival rates in hours and h were assayed with K method and the cell apoptosis and cycles
.Results 唱唱
with the FCM methods The cell growth was greatly inhibited by allicin in a concentration and time
(P <0畅001). 0 /1 .
dependent manner FCM results showed that cells were predominately blocked in the G G phases The
( P <
apoptosis rate was significant