ms易感基因 认知障碍与细胞免疫治疗机制的分析.docx

Abstract
Multiple sclerosis (MS) is a T cell mediated autoimmune disease with the involvement of ic and environmental factors. Natural killer (NK) cells are critical regulators of innate and adaptive immunity. NK cells from MS patients decreased in number and were defective in cytokine production. As many diseases were associated with NK cell defection, it was largely unknown whether the alteration of NK cells in number and function preceded the development of MS or resulted from MS. Given MS is a ic susceptible disease, we explored KIR (killer immunoglobulin-like receptor) and their HLA-C (human leukocyte antigen) ligand genes in the first part of our experiment. RT-PCR was used to detect the genotype of KIR and HLA-C in MS patients and controls. Odds Ratio risk test was applied to evaluate the risk of KIR/HLA-C genes in MS. We found that MS patients with higher frequency of inhibitory KIR2DL2 had increased risk of MS. The sole existence of HLA-C1 and HLA-C2 ligand gene did not increase the risk of MS. Carriers of HLA-C1 ligand gene had a higher risk for MS than noncarriers among individuals also carrying KIR2DL2 gene, which suggested KIR2DL2 execute its functions mainly bination with HLA-C1 ligand.
MS manifested variable symptoms and signs according to the disparate lesions. 40%-70% MS patients acquired cognitive impairment, it could appeare early in the course of MS. However, the cognitive insult was always ignored because of the prominent motor disability, early detection and treatment of cognitive impairment would improve the quality of life in MS patients. In the second part of our experiment, Digit Symbol Modality Test (DSMT), Semantic Verbal Fluency Test (SVFT) and Digit Span Test (DST) were used to evaluate the cognitive functions in RRMS patients. We found impairment in information processing speed, memory, sustained attention and executive function in MS patients. Corpus calllosum (CC) is the largest white matter bundles connecting both hemispheres and