CHEMICAL CONSTITUENTS OF Ficus odorata.pdf

Pharmaceutical Chemistry Journal, Vol. 46, No. 4, July, 2012 (Russian Original Vol. 46, No. 4, April, 2012)
CHEMICAL CONSTITUENTS OF Ficus odorata
P.-W. Tsai,1 K. A. De Castro-Cruz,1 C.-C. Shen,2 C.-T. Chiou,2
and C. Y. Ragasa3,4,*
Original article submitted February 03, 2012.
A dichloromethane extract of the leaves of Ficus odorata (Moreaceae family) contains b-sitosteryl-
3b-glucopyranoside-6¢-O-palmitate (1), squalene (2), lutein (3), a-amyrin acetate (4), lupeol acetate (5), and
b-carotene (6). The structure of 1 was elucidated by extensive 1D and 2D NMR spectroscopy. Compound 1
was tested for cytotoxicity using sulforhodamine B assay against the human lung adenocarcinoma epithelial
cell line (A549), human stomach adenocarcinoma cell line (AGS), human colon adenocarcinoma (HT29) and
human prostate cancer cell line (PC3). It exhibited cytotoxicity against AGS cell line with % growth in-
hibition
Key words: Ficus odorata, Moreaceae, b-sitosteryl-3b-glucopyranoside-6¢-O-palmitate, cytotoxicity
INTRODUCTION EXPERIMENTAL
Ficus odorata (Moreaceae family), locally known as Materials and Methods
pakiling, is an endemic medicinal tree in the Philippines,
which grows in secondary forests at low altitudes [1]. It is an General experimental procedures. The 1H NMR and
ethnomedicinal plant used by the Aetas of Bataan, Philip- 13C NMR spectra were recorded