实验论文 (8).doc

鹿茸多肽对大鼠软骨细胞增殖的影响
作者:陈晓东林建华作者单位:巴彦淖尔市医院骨科,内蒙古巴彦淖尔 015000;福建医科大学附属第一医院
【摘要】目的:探讨鹿茸多肽(PAP)对大鼠软骨细胞体外增殖的影响。方法:采用二次酶消化法获得大鼠关节软骨细胞进行传代培养并对第四代细胞进行药物干预,利用MTT比色、免疫组化检测增殖细胞核抗原(PCNA)、流式细胞术检测细胞周期等方法来检测各代次大鼠软骨细胞和药物干预后第四代软骨细胞的增殖情况。结果:大鼠软骨细胞随传代培养,MTT比色显示增殖呈下降趋势,A表达减少,流式细胞术显示细胞增殖指数下降;PAP可以抑制这一趋势,促进传代培养的细胞增殖能力。结论:PAP对传代培养的大鼠软骨细胞的增殖能力有明显促进作用。
【关键词】鹿茸多肽;大鼠;软骨细胞;增殖
基金项目:福建省科技计划重大资助项目(2004Y018)
Effect of Pilose Antler Polypeptides on Proliferation of Rat Chondrocyte in Vitro CHEN Xiaodong,LIN Jianhua (Department of Orthopedics,Bayannaoer Hospital,Bayannaoer 015000,China)
Abstract Objective: The purpose of the present was to investigate the effect of pilose antler polypeptides(PAP) on the proliferation of rat chondrocyte in : The rat articular chondrocytes were isolated by enzyme digestion and sub-cultivated in 4th chondrocyte was interfered with chondrocytes of different generations and the 4th medicine intefered generation were detected with the methods of proliferating cell nuclear antigen , MTT(methyl thiazolyl tetrazolium) assay for proliferation and Flow cytometry for analyses of cell cycle distribution and PI(proliferation index).Results: With chondrocytes passage time increasing, the proliferating ability of chondrocytes assay showed that the proliferating ability of chondrocytes decreased, the expression of PCNA tapered in the experiment