cnos和inos在肉鸡肺动脉高压综合征中的作用研究-临床兽医学专业毕业论文.docx

华中农业大学2005届硕士学位论文
差异显著(P<);(3)K+浓度在给药1h和2h后显著或极显著低于对照组(P<0,01或P<0,05)。试验二的研究结果表明L AG通过抑制iNOS活性,引起***mPAP的升高,从而推测iNOS具有调节***体内mPAP的作用并与PHS 发生有着密切的联系。
关键词:一氧化氮:诱导型一氧化氮合酶:原生型一氧化氮合酶;L-氨基胍肺动脉高压综合征;***
cN0s和iNOS在***肺动脉高压综合征中的作用研究
The Effect of eNOS and i NOS on Pu I monary Hypertens i OR
Syndrome i n Broi Iors
ABSTRACT
The pulmonary hypertension syndrome in broilers(PHS),which is also called ascites syndrome of broilers(ASB),has a group of clinical symptoms such as obvious ascites,right heart enlarged,lung congestion,hydroncus and so disease is
ofen occurred in 21-day-old and 28-day—old incidence of the disease is more than 60%,the mortality is even up to 42%in some of scholars consider that the key of PHS is pulmonary hypertension that is caused by the damage of homeostasis between the vascular relaxing factors and the vascular constricting oxide(NO),which is a reprehensive of vascular relaxing factors,is
produced by L·Arg under the function of aim of this research is to throulglI
two experiments explain the function of constitutive OS)and inducible NOS (iNOS)in PHS:(1)In the experiment one,iNOS OS activities in lung and serum of PHS and ascites broilers(group S).PHS broilers(group M)and health broilers(group C)were measured at the age of 21,28,35 and 42 days。AHI, WH/Bwj mPAP and NO in serum were measured as well;(2)In the experiment two, the broilers were divided into two groups which were given physiological saline and L·AG respectively through intravenous injection,and measured mPAP,superoxide dismutase(SOD),catalase(CAT),iNOS and eNOS activities,NO level,PCV electrolyte level,pH,and malondialdehyde(MDA)level at 1,2 and 4 hours after the broilers were administrated results of experiments were as follow:(1)
From the age of 28 days,WH/BW of group S and group M and NO level in serum of
group S are higher or significantly higher than that of group C(P< or P<); (OS activity in lung of grou