csa和tnf-α对体外培养gfs增殖和胶原代谢的影响-口腔临床医学专业毕业论文.docx

硕士学位论文 中文摘要
^与TNF—a共同作用于成纤维细胞时,促进成纤维细胞的增殖。提示在~定浓度下CsA可能放大TNF-Q刺激成纤维细胞增殖的效应。
-a共同作用于成纤维细胞时,促进胶原含量的增加。提示TNF-a可能增加了成纤维细胞对Csh的敏感性。
关键词牙龈成纤维细胞,CsA,TNF—a,牙龈增生
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硕士学位论文 ABSTR ACT
ABSTRACT
Objective:Study the effect of cyclosporine A(CsA)and tumor necrosis factor Q(n师-a)on cell proliferation and collagen biosynthesis of cultured human gingival fibroblasts(GFs),to found the relationship between gingival inflammation and drug—induced gingival overgrowth,and the mechanisms of gingival overgrowth induced by CsA. Methods:Human GFs were cultured in vitro using a method of tissue
cells舶m the 4th~8th passage were used in
were cultured and incubated with various concentrations of CsA and
TNF一0(A:blank group,BI:10 ug/L Cs八B2:50 ug/L Cs八B3:250 ug/L Cs八B4:1250 ug/L CsA,C:5 ug/t,TNF-Q,DI:10 ug几CsA+5 ug/L n旺-Q,D2"50 ug/L CsA+5 ug/L TNF—Q,D3:250 ug/L CsA+Sug/L TNF—a,D4:1250uglL CsA+5 u#L TNF—Q)solution for 3,5, assay and hydroxyproline reagcuat were used to evaluate the cell proliferation and collagen production in the culture meidiun.
Result:The ess rate of primary culture of human gingival
fibroblasts was up to 80%,the cells were spindle—shaped,arranged in trabs or cells had no morphological changes after exposure.
、阴崦n exposure to CsA of different concentration,the proliferation of fibroblasts was value B1,B2,B3 had nO significant difference丽th control group,while the A value of group B4
Was significantly higher than control group(P<).Fibroblast proliferation was significantly increased while cultured with 5ug/L value increased(P<).When exposure to CsA+,A value of group Dl、D2、D3 were much higher than group八but were lower than group proliferation in group 1)4 was significantly increased,the difference with group C had statistical significance.
、Ⅳhen exposure to CsA of different concentration,collagen content in.
supernatant was increased,and the gro