—— 一 一 奎堕查兰婴圭兰堡笙苎 The Expression of CTGF in Pulmonary Vessels of Hypoxemic Pulmonary Hypertensive Mouse Department of Respiratory Medicine,ZhongDa hospital affiliated of Southeast University,Nanjing,210009,China Graduate:Zhang Qiang,Supervisor:Lin Yong Abstract OBJECTIVE:A model of hypoxemic pulmonary hypertensive mouse was conducted to explore the expression of connective tissue growth factor(cTGF)in the pulmonary arteriole from pulmonary hypertensive :The modeI of hypoxernic pulmonary hypertensive mouse was constructed by a method of interval ordinary pressure cardiac catheterization was employed for mPAP detection.,and RV/(LV+S)was determined by tissue section staining and image processing detected the morphologic change of pulmonary arteriole. CTGF Ievels in the pulmonary arteriole were determined by immunohistochemistry. and the concentrahon was measured by image pattern analysis and semi—quantitative :Three weeks results of naPAP、 RV/(LV+s),and the levels of U玎%and WA%indicating the proliferation of vessel wall in the group of hypoxemic group are higher than those in normal group fp<)There is no expression of CTGF protein in the pulmonary arteriole of the nortonl ,it was highly expressed in the tunica adventitia vasorum, 51ightly expressed in the endangium and seldom expressed in the tunica media vasorum of pulmonary arteriole in the hypoxemic group. Keywords:Hypoxia;pulmonary vascular remodeling;connective tissue growth factor U 东南大学学位论文独创性声明 本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知,除了文中特别加以标注和致谢的地方外,论文中不包含其他人已经发表或撰写过的研究成果,也不包含为获得东南大学或其它教育机构的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了谢意。 {帚 研究生签名: 筮萱 日 期: 2QQ5:业:!鱼 东南大学学位论文使用授权声明 东南大学、中国科学技术信息研究所、国家图书馆有权保留本人所送交学位论文的复印件和电子文档,可以采用影印、缩印或其他复制手段保存论文。本人电子文档的内容和纸质论文的内容相一致。除在保密期内的保密论文外,允许论文被查阅和借阅,可以公布(包括刊登)论文的全部或部分内容。论文的公布(包括刊登)授权东南大学研究生院办理。 1索 搬 吒 研究生签名: 瑟董导师签名 挞曼日 期:2QQ』:Q3二!互 东南大学硕士学位论文 绪 言 慢性阻塞性肺病(chro