硕士学位论文 ABSTRACT OBJECTIVE:To explore four polymorphisms of CYP2A6(1,2,3 and 4)for association with o metabolin related coronary heart disease;to explore a convenient method to optimize PCR conditions. METHODS:The genomic DNA was extracted from 205 patients 、衍tll essential coronary heart disease(CHD)and 208 health adult Han subj ects(NC)in on their smoke habit,they were devided into smokers(SM)and nonsmokers groups(NS),207 and 206 subject analysis was Design was applicated to optimize the four factors that may affect the polymerase chain reaction(RCR).The polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism(RCR-RFLP). RESULTS:(1)The most favorable PCR conditions of CYP2A6 was: annealing temperature 6 ℃,DNA template content 50ng,the concentration of M92+ mmol/L,Taq polymerase contents .(2) The CYP2A6 genotypic frequency did not deviate from Hardy-Weinberg equilibrium in CHD and NC was no significantly difference of the distributions of four genotypes between the CHD and NC groups.(3)The CYP2A6 genotypic frequency did not deviate from Hardy—Weinberg equilibrium in SM and NS frequency was higher in SM group than in NS group(SM:%VS NS:%,P< X2=).CYP2A6*4 frequency was higher in NS group than in SM group(SM: 1%VS NS:%,P< X2=). Morbidity of CHD was higher in SM group than in NS group(SM: %VS NS:%,X2=,P<). CONCLUSION:(1)Uniform Design can act as a powerful tool for optimizing PCR reaction conditions.(2)CYP2A6 polymorphism may effect individual smoke habit.(3)Smoke may be a risk factor of coronary artery disease.(4)There was no correlationship between CYP2A6 polymorphisms and o metabolin related cardiovascular disease. KEY WORDS:CYP2A6,uniform desigin,coronary heart disease, ic polymorphism,PCR—RFLP lI 硕士学位论文 中英文词汇对照 中英文词汇对照 cardiovascular disease,CD 心血管病 cerebrovascu