低硒日粮对肉鸡免疫器官中硒蛋白与细胞因子表达的影响-兽医学；基础兽医学专业毕业论文.docx

CoNTENTS
Abstract in I::hinese . l Abstract in English ... . . . . . . . . . . . . . . . .. . . . . .. III
1 Intruduction ..1
Selenium and Selenoproteins . . . ..1 The classification, function and location of seleno口roteins ..1 The regulation of selenium to selenoprotein in tissues 4
The effects of selenium defiency on ans ..4 The effects of Se defiency on ans morphological structures .4 The effects of selenium defiency on immune functions ..5
】 Purpose and significance ofthe study.. ... ... . . . . . . . . . . .. ..6
2 Materials and methods .7
:!.1 Main instruments and reagents. . .7 Main instruments ..7
Main reagents .7
Experimental animal and the ED broilerc chickens modelling ..8
Experimental animal group and feeding .8
Tissue samples collection and handling methods 9
Detection indexes and method of experiment .9
Observation ofhistopathology 9
Total RNA extraction in the immune tissues and eDNA synthesis .9 23 kinds of seleniumprotein mRNA expression in immune tissues .11 Detectionthe mRNA Ievels ofcytokines in ans ..13 The experimental data statistical analysis 1:;
3 Results and alialysis................ ... . ... ............................... . . .. . ..14 Pathological observation ..... . ... . .... .. . . . . . . . . . . .. .14 The rsult ofobserval[ion in spleen ..14
The rsult ofobservation in thymus .15
The rsult ofobservation in bursa ofFabricus 17
The results of Se deficiency on Sels gene expressions in immune tissues .18 The results of Se deficiency on Sels gene expressions in immune tissues l 8 The results of23 Sels expression patterns in broiler ans .29 The effects of Se deficiency on cytokines expressions in ans .29 The effects of Se deficiency on IL-4 expression in ans 29
The effects of Se deficiency on IL-6 expression in ans 29
The effects of Se deficiency on IL-10 expression