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低夜温下黄瓜雌性分化相关代谢与蛋白质变化的初步分析-园艺学;蔬菜学专业毕业论文.docx


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文档列表 文档介绍
CONTENTS
CoNTENTS
Abstract in Chinese .I
Abstract in English... ........ .. ... ...... . ...... -.. III
1 Introduction 1
Purpose and significance 1
National and abroad literature summarization 。1
ic basis of sex determination in Cucumber 1
The physiological and biochemical basis of sex differentiation in cucumber and the influence ofenvironment .2
The transcriptional study of sex determination in Cucumber . . .......5
Advances in proteomics research .. 6
Existing problems and prospects .......... . ......... .......... ...... ...-..6 Research contents .............. .. ............. ............. ............ ..7 Main research contents ..7
Technology routes . ............ . ............ .................... ....... .7 2 Materials and methods 8
Experiment matedals 8
Plant cultivation and temperature treatments 8
Sample preparation........... ..................... . .................... . ............. ..8 Determination ofmetal)olism related indexes ..8
Determination of sugars .8
Determination ofhormones and amino acid ..10
Determination ofrelated enzymes ..1 1
Proteome difference analysis....... .. ................ .... ............................ ....12
Separation ofprotein samples......... . ....... ............. . . ...12
Determination ofprotein concentration by bradford............ . . . . . ...............1 3
2-DE two-dimensional gel electrophoresis................ .... . .............14 Gel dyeing........... . ..................... . ... ...............16
Gel scanning and image analysis.................. .... . . ... ..... ......................... 16
Mass spectrometry analysis................ ... . .. . ................................ .1 7 Verification ofprotein differential point in transcription level. . .... . ... .............17 Extraction ofRNA .17
Synthesis ofcDNA first chain ..17

低夜温下黄瓜雌性分化相关代谢与蛋白质变化的初步分析-园艺学;蔬菜学专业毕业论文 来自淘豆网www.taodocs.com转载请标明出处.

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  • 页数60
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  • 时间2018-06-25