PCR（PCR）.doc

PCR（PCR）.docPCR(PCR)
Experiment twenty-three PCR diagnostic techniques for parasitic diseases of animals
Polymerase chain reaction (Polymerase Chain Reaction PCR) technology is a sensitive and specific DNA in vitro amplification method, it can be a short to DNA amplification of millions of times, the amplification efficiency so that the method can detect trace DNA single worm or only part of the worm. By designing species and strains specific primers, this method only amplified the specific PCR products of plant species and strains with high specificity. And the operation process of PCR technology is relatively simple, no need for pathogen isolation and purification; at the same time can e the interference of antigen and antibody persists, direct detection of pathogens of DNA, which can be used for the identification of species and strains, the clinical diagnosis of animal parasitic diseases, but also can be used for molecular epidemiological investigation of animal parasitic diseases. With the continuous improvement and development of PCR technology, it has been widely used in molecular biology, biotechnology, clinical medicine and other fields, and has a wide range of applications.
I. requirements of the experiment
The basic principle of master PCR diagnosis technology involved in the experiment, the operation process of fully familiar with PCR diagnostic techniques, including parasites DNA extraction, PCR primer design, PCR condition optimization, fragment amplification, agarose gel electrophoresis and the result analysis, PCR purification and so on.
Two 、 experimental instruments and materials
(1) extraction and purification of genomic DNA from parasites
1. worm material. Individual worm.
2. implements. Clean bench, incubator, TGL-16G centrifuge, vortex oscillator, trace liquid taking device and auxiliary suction head, microcentrifuge tube, disposable syringe, micro ophthalmic forceps, micro ophthalmic scissors, glass plate, dropper, rack etc..
3. reagent.
(1) (Et