温莪术药材指纹图谱的研究.doc

温莪术药材指纹图谱的研究
方丽1,祝明2*,郑成2,张鹏1
(1. 浙江中医药大学药学院,浙江杭州 310053 2. 浙江省食品药品检验所,浙江杭州 310004)
摘要目的:建立温莪术的HPLC指纹图谱分析色谱条件,拟定温莪术指纹特征图谱的共有模式。方法:采用kromasil C18色谱柱;流动相为乙***-%磷酸(梯度洗脱) ;检测波长为216 nm;柱温为30℃;流速为1ml/min。结果:温莪术共标示出13个共有峰。10批温莪术相似度较好,。用本文建立的测定方法,能够明确区别温莪术和广西莪术及蓬莪术。结论:方法准确可靠,重复性好,为温莪术质量控制提供了保证。
关键词温莪术;指纹图谱; HPLC
Fingerprint Analysis of Curcuma wenyujin et
Fang Li1,Zhu Ming2,Zheng cheng2,Zhang peng1
(1,College of pharmacy, Zhejiang University of Traditional Chinese Medicin,Hangzhou,Zhejiang,China 310053 2,Zhejiang institute for food and drug control,Zhejiang Hangzhou 310004)
ABSTRACT: Objactive To establish a method of fingerprint analysis of Curcuma wenyujin et by HPLC. Method HPLC was performed on an kromasil Cl8 analytical column (416 mm×250 mm, 5μm) with gradient elution (0 - 45 min, 45% A→95% A; 45 - 55 min, 95% A) of acetonitrile (A) – % phosphoric acid (B) at the flow rate of mL/min. The column temperature was 30 ℃and UV detection was performed at 216 nm. Result 13 peaks were identified as the characteristic fingerprints of Curcuma wenyujin et and the fingerprints of the 10 samples had high similarities of different species of Curcuma were method of fingerprint chromatogram with characteristic and specificity will be used to identify Curcuma of different species. Conclusion:The develo