丁苯酞对血管性痴呆大鼠记忆能力及海马CA1区Caspase―3表达的影响.doc

丁苯酞对血管性痴呆大鼠记忆能力及海马CA1区Caspase―3表达的影响

[摘要] 目的观察丁苯酞对血管性痴呆大鼠记忆能力及海马CA1区凋亡基因Caspase-3表达的影响,探讨丁苯酞对血管性痴呆的保护作用。方法将80只SPF级健康Wistar大鼠按随机区组法分为4个组,每组20只:血管性痴呆模型组(VD组)、血管性痴呆模型+丁苯酞***化�c注射液组(NBP治疗组)、假手术+丁苯酞***化钠注射液组(NBP对照组)、假手术组(Sham组),每组再分为4个亚组:术后1、2、4、8周,每个亚组5只。永久性双侧颈总动脉结扎法制备VD大鼠模型。NBP对照组和NBP治疗组大鼠术后1 d开始给予丁苯酞***化钠注射液腹腔注射,剂量为5 mg/(kg・d),Sham组和VD组大鼠给予生理盐水腹腔注射( mL/d)。各组大鼠连续腹腔注射给药7 d。术后8周亚组大鼠,在术前、术后4周和术后8周进行记忆能力测试(Morris水迷宫)。术后各时间点(1、2、4、8周),留取海马组织,采用免疫组化法观察各组大鼠海马CA1区Caspase-3的表达。结果 NBP治疗组大鼠逃避潜伏期较VD组大鼠明显缩短(P 中国论文网/6/view-
[关键词] 丁苯酞;血管性痴呆;海马;细胞凋亡
[中图分类号] R743 [文献标识码] A [文章编号] 1673-7210(2017)10(a)-0009-04
[Abstract] Objective To study the effects of 3-n-butylphthalide (NBP) on memory and Caspase-3 expression in the hippocampal CA1 region of vascular dementia rats, in order to explore protective effects and mechanisms of NBP on rats with vascular dementia (VD). Methods A total of 80 SPF Wistar rats were divided into vascular dementia models group (VD group), vascular dementia models + NBP injection group (NBP treatment group), sham surgery + NBP injection group (NBP control group), sham surgery group (Sham group), according to random group method. Then each group was divided into four subgroups (n = 5): 1, 2, 4, and 8 weeks after surgery. VD models were established by ligating mon carotid artery. Then the rats in the NBP treatment group and NBP control group were intraperito