A Practical Handbook Of Preparative Hplc.pdf

1/19/06 9:44 AM Page i
A Practical
Handbook of
Preparative HPLC
1/19/06 9:44 AM Page ii
Don Wellings is the Chief Scientific Officer
for Chromatide Ltd, pany specializing
in contract purification and consultancy
services. Don has been performing prepar-
ative chromatographic separations since his
PhD where he was routinely running 10 cm
diameter columns more than 20 years ago.
The wealth of knowledge accumulated over
the essive years has been as broad as it is deep.
Prior to setting up Chromatide with an ex-colleague from
Avecia, he was Technology Manager for Special Projects at
Polymer Laboratories, where he was intimately involved in the
design and development of new polymeric stationary phases
for reversed phase, normal phase, ion-exchange, affinity and
chiral HPLC. Previously Don was Technology Manager for
Separation Sciences and Solid anic Chemistry at
Avecia. During this period he developed an expertise applying
molecular modeling to the design of chiral ligands for prepar-
ative chromatography.
In the late 1990s he was involved in the installation -
missioning of process scale HPLC at Zeneca Pharmaceuticals.
During 18 years with CRB, ICI, Zeneca and Avecia he was
instrumental in establishing the technique of preparative
HPLC within pany and served 11 years as the secretary
of pany’s Process Scale Chromatography Group.
1/19/06 9:44 AM Page iii
A Practical
Handbook of
Preparative HPLC
Dr. Donald A. Wellings
AMSTERDAM • BOSTON • HEIDELBERG • LONDON • NEW YORK •
OXFORD • PARIS • SAN DIEGO • SINGAPORE • SYDNEY • TOKYO
1/19/06 9:44 AM Page iv
Elsevier
The Boulevard, Langford Lane, Kidlington, Oxford, OX5 1GB, UK
Radarweg 29, PO Box 211, 1000 AE Amsterdam, herlands
Copy © 2006 Elsevier Ltd. All rights reserved
The right of Don Wellings to be identified as the author of this work has been asserte