表没食子儿茶素没食子酸酯抑制地塞米松体外诱发兔白.doc

表没食子儿茶素没食子酸酯抑制地塞米松体外诱发兔白.doc表没食子儿茶素没食子酸酯抑制地塞米松体外诱发兔白
【摘要】目的:观察表没食子儿茶素没食子酸酯(EGCG)对抑制地塞米松(Dex)诱导体外培养兔晶状体上皮细胞(LEC)凋亡的作用。方法:采用体外培养正常家兔晶状体,随机将家兔晶状体分为3组:空白对照组(A);Dex(10μmol/L)处理组(B);Dex+EGCG(10mmol/L)实验组(C)。分别于3,5,7d培养结束后观察各组晶状体的透明度,并进行相关比较及统计学分析。TUNEL法检测EGCG对Dex诱发的兔LEC凋亡细胞数量的影响,并进行相关比较及统计学分析。结果:EGCG实验组晶状体透明性均低于空白对照组,但高于Dex处理组,差异有显著统计学意义(P<);Dex处理组 LEC凋亡率显著高于空白对照组(P<);Dex处理组与EGCG实验组比较,有显著性差异(P<)。结论:EGCG可抑制Dex损伤所诱导兔LEC调亡,具有明显抑制激素性白内障形成的作用。
【关键词】表没食子儿茶素没食子酸酯;白内障;地塞米松;晶状体上皮细胞;凋亡
AbstractAIM: To observe the inhibitory effect of epigallocatechin gallate(EGCG) on apoptosis of lens epithelial cell(LEC) of rabbits dexamethason(Dex)peroxide : Cultured normal rabbit lens in vitro ly divided into 3 groups: the control group(A),the group treated by Dex(10μmol/L)(B),the group treated by Dex peroxide and EGCG(10mmol/L)(C).The transparence and apoptosis ratio of lens on the time points of 3,5,7 days ined by image analysis and floetry,as inal deoxyribonucleotide transferasemediate dUTP nick end labeling(TUNEL)detection, the effect of EGCG on the number of dexamethason induced rabbit LEC ined,the related parisons and statistical analyses ong groups ethod revealed that the apoptosis rate of LEC in Dex group ent of the cataract.
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KEYA公司);DMEM培养基(Hyclone公司);TUNEL试剂盒(武汉boster公司)。正常成年家兔50只,经耳缘静脉注射空气形成栓塞致死,立即摘取眼球于超净工作台后路法小心取出晶状体,清除干净玻璃体,置入含青霉素80万U+庆大霉素8万U的生理盐水500mL中,1min后取出置入5mL DMEM低糖培养液(含100mL