张荣娟差异基因.ppt

代号
基因
推断功能
同源性
RJ2
Conserved protein(氧化还原酶位于同一个操纵元中,还没有关于此基因的研究)
98%
RJ4
secA
Probable preprotein translocase SecA subunit functions in protein export;
74%
RJ6
CgtA和ProB1
Putative GTP-binding protein和Probable glutamate 5-kinase
98%
RJ7
prkA
PrkA; Putative Ser protein kinase(Signal transductionmechanisms)
76%
RJ9
gltA
type II citrate synthase:(Citrate synthase activity was essential for nodule maintenance )
70%
RJ14
thiC
putative thiamine biosynthesis protein
68%
RJ5
fix
RJ12
Transcriptional regulator, LysR family
与1021相似性低但与其他物种比对也是这个蛋白
总结----6个差异基因
Q-PCR验证部分差异基因
RJ4 secA
RJ6 CgtA
RJ6b ProB1
RJ7 prkA
RJ9 gltA
RJ14 thiC
总结
a
b
a
a
b
c
b
a
c
b
a
b
a
a
a
a
b
b
b
b
b
a
c
c
c
JY9(gltA)
文献报道:
根瘤菌突变体中:不能结瘤或形成无效瘤,类菌体减少,胞外多糖比野生型多三倍,推测破坏了侵染线,缺乏能量,对结瘤固氮必须;
大肠杆菌中:突变体生长速度减慢。
a
b
c
JY14---thiC
文献报道:
Putative thiamine biosynthesis protein ;
突变体会推迟结瘤;
Thiamine is a role during the transition from aerobic to fermentative-like metabolism
b
a
JY4(secA)
文献报道:
细菌特有的一种蛋白质,具有ATP酶活性,是S e c蛋白质转运途径中的“动力泵”,通过 ATP的水解循环驱使多肽穿过通道。
c
b
a
JY7(prkA)
文献报道:
丝氨酸蛋白激酶;仅在芽孢杆菌中有报到;是一种cAMP依赖的蛋白激酶作用。
c
b
a
JY6B和JY6(下调)
Disruption of the cgtA gene was lethal, demonstrating that this gene is essential for cell growth.
secA
and Bacillus 功能已经明确, hydrolyzes ATP and an ponent of the protein translocation ATPase ;
SecA Protein的ATP-binding site已经确定;
SecA 识别并结合 preprotein-plex, 结合到 inner-membrane anionic phospholipids, 插入 the membrane bilayer 以后,再与the preprotein translocator, SecY/SecE结合,从而将蛋白运输过膜.
putative transport protein transmembrane
hypothetical protein
c
b
a
SecB