生物技术毕业论文.doc

摘要
红掌(拉丁名:Spathiphyllum floribundum),亦叫安祖花,红鹤芋等,天南星科火烛属。其花色艳丽,花茎挺拔,叶型翠绿美观,是当今世界著名的切花和盆栽花卉之一,市场潜力大,具有极高的观赏价值和经济价值。红掌的繁殖方法通常采用分株繁殖、扦插繁殖和组织培养繁殖。但分株繁殖、扦插繁殖很难满足当前市场的需求,组织培养是红掌快速繁殖的有效途径。由于组织培养中经常出现不同程度的污染问题,造成很大的损失,因此控制污染是植物组织培养苗工厂化生产中重要的技术环节。本文从灭菌方法、内源性污染、继代培养中污染防治等方面,进行了红掌组培快繁过程中污染原因的分析及对策的探讨()。

关键词:红掌,组织培养,灭菌,污染,防治
Andraeanum during tissue culture analysis of the causes of pollution and related countermeasures
ABSTRACT
Anthurium, also called Andrew Flower, lines such as flamingo, a Fire Araceae. Its bright colors, tall and straight stem, green leaf-type appearance, are well-known in today's world of cut and potted flowers, one large market potential, with high ornamental value and economic value. Flower andraeanum breeding monly used ramets propagation, cutting propagation and tissue cultur e propagation. However, ramets propagation, cutting propagation is difficult to meet current market demand, tissue culture andraeanum Flower Express are an effective way of breeding. Tissue culture because of the frequent occurrence of various degrees of pollution, resulting in great loss, so control of pollution are industrial plant tissue culture ine production technology in the important aspect. This article from the sterilization method, endogenous pollution subculture in such areas as pollution prevention, to discuss how to minimize the emergence of tissue in the pollution problems.


KEY WORDS: Anthurium,tissue culture, sterilization, pollution,prevention and treatment
目录

前言 1
第1章培养基的灭菌 2
2
2
第2章外植体材料的消毒与灭菌 4
4
第3章接种前的准备工作 5
、工具的灭菌 5
5
5
5
5
第4章红掌诱导与继代培养过程中的污染 7
第5章污染的原因和预防措施 8
8
8
8
9
9
9
10
10
10
结论 11
谢辞 12
参考文献 13
附录 15
外文资料翻译 18

前言

红掌(拉丁名:Spathiphyllum floribundum),天南星科火烛属,原产地哥伦比亚。其花色艳丽,花茎挺拔,叶型翠绿美观,是当今