电针足三里对AD小鼠大脑皮层APP和Aβ表达的影响.pdf

倡天津市高等学校科技发展基金项目(20090206)礂天津中医药大学第一附属医院病理科(天津 300193)▲天津市针灸研究所(天津 300193)电针足三里对 AD 小鼠大脑皮层 APP 和 Aβ表达的影响倡李 翀礂 韩燕燕礂 樊小农▲ 天津中医药大学(天津 300193) 摘 要 目的:探究电针足三里对老年痴呆小鼠大脑皮层β‐淀粉样肽前体蛋白(APP)及β‐淀粉样肽蛋白(Aβ)表达的影响。方法:将快速老化小鼠 SAM P8 随机分为模型组、电针组和非穴组,并设同源 SAMR1 小鼠作为对照组。电针组给予足三里电针干预,非穴组针刺双侧固定非穴位点。应用免疫组织化学 SABC 法观察各组小鼠大脑皮层 APP 及 Aβ的表达情况。结果:SAMP8 大脑皮层内 APP 和 Aβ蛋白呈较高表达的状态,电针治疗后明显下调,与模型组、非穴组相比,差异有统计学意义(P< 0 .01) ;而非穴组与模型组相比,各指标差异无统计学意义(P> 0 .05) 。结论:电针足三里能有效改善 AD ,其机制可能与其下调 AD 小鼠大脑皮层 APP 及 Aβ蛋白水平的表达有关。 主题词 阿尔茨海默病/针灸疗法 电针 穴,足三里 动物实验 小鼠 【中图分类号】 R749 【文章标识码】 A doi :10 .3969/j .issn .1000‐7369 .2015 .05 .049Effects of electro‐acupuncture at “Zusanli” on the expressionsof APP and Aβ in the cortex of Alzheimer's disease miceTianjin College of Traditional Chinese Medicine(Tianjin 300193) Li Chong Han Yanyan Fan Xiaonong ABSTRACT Objective :To investigate the expressing levels of APP and Aβ proteins owing to the influence of e‐lectro‐acupuncture at “Zusanli” in Seneseenee accelerated mice (SAMP8 ) brain .Methods : The Seneseenee acceleratedmice (SAM P8) were randomLy divided into 3 groups :model group ,anelectro‐acupuncture group ,and sham‐acupuncturegroup .The SAMR1 was used as control .The expressing levels of APP and Aβ proteins were evaluated by immunohisto‐chemical assays .Results :The expressing levels of APP and Aβ proteins in the cortex of Seneseenee accelerated mice werehigh