慢性阻塞性肺疾病患者肺内细胞凋亡研究.doc

慢性阻塞性肺疾病患者肺内细胞凋亡的研究
作者:石青作者单位: 重庆市铜梁县人民医院呼吸内科
【摘要】目的探讨慢性阻塞性肺疾病(COPD)患者肺内细胞凋亡情况,以其为临床治疗提供治疗靶点。方法收集COPD患者和非COPD患者肺泡灌洗液,进行细胞培养,取上清液测定白介素8(IL8)和肿瘤坏死因子Α(TNFΑ)含量,测定细胞凋亡率。结果 COPD组细胞培养上清液中IL8和TNFΑ含量明显高于对照组(P<);COPD组肺泡巨噬细胞的凋亡明显高于对照组(P<)。结论 COPD患者存在着强烈的炎性反应和细胞凋亡情况,可能是患者治疗靶点。
【关键词】肺疾病,慢性阻塞性;细胞凋亡;白细胞介素8;肿瘤坏死因子Α
【Abstract】 Objective To investigate the apoptotic state of pulmonary cells in patients with chronic obstructive pulmonary diseases (COPD) in order to provide therapeutic targets for clinical therapy. Methods The alveolar irrigating solutions of COPD patients and nonCOPD patients were collected,and the cell culture was performed,then the supernatant liquid was taken out to detect the contents of IL8 and TNFΑ,and the cell apoptosis rate was also detected. Results The levels of IL8 and TNFΑ in the supernatant liquid of cell culture in COPD group were significantly higher than those of control group(P<).The apoptosis rate of alveolar macrophage in COPD group was significantly higher th
an that of control group(P<). Conclusion The patients with COPD exist intensive inflammatory reaction and apoptosis,which may be a novel therapy target.
【Key words】 pulmonary diseases,chronic obstructive; cell apoptosis; IL8; TNFΑ
慢性阻塞性肺疾病(COPD)是一种呼吸系统常见疾病,具有较高的患病率和病死率。病理学研究表明,COPD患者存在着肺内细胞增殖和细胞死亡的平衡失调[1,2]。本研究对我院收治的COPD患者及非COPD患者,采集肺泡灌洗液,进行肺内细胞凋亡检测及相关分析,为发病机制及治疗提供