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加盟商店铺运营技巧.ppt


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miR-18a对A549细胞的放射增敏作用及分子机制研究
吴磊1,孙建国1,徐睿1,李德志1,徐燕梅1,马虎2,李雪涛1,彭丽娜1,陈正堂1(400037重庆,第三军医大学新桥医院全军肿瘤研究所1;563003 贵州遵义,遵义医学院附属医院肿瘤医院2)
[摘要] 目的观察miR-18a对A549肺腺癌细胞放射敏感性的影响并初步探讨其机制。方法 qRT-PCR检测2Gy X线照射A549细胞后miR-18a的变化;将人工合成的miR-18a模拟物(mimics)转染A549细胞,测定不同剂量照射后A549细胞的克隆形成能力,描记细胞存活曲线,测算2Gy时存活分数(SF2)等指标,判断miR-18a对A549细胞放射敏感性的影响;同时检测过表达miR-18a的A549细胞中共济失调-毛细血管扩张症突变基因(Ataxia-telangiectasia mutated,ATM)蛋白,探讨miR-18a对A549细胞放射敏感性影响的分子机制。结果与照射前(±)相比,2 Gy剂量照射后A549细胞miR-18a水平(±~±)显著降低(P<)。miR-18a过表达后A549细胞放射敏感性增强,miR-18a过表达组、,;同时miR-18a过表达的A549细胞中ATM、磷酸化ATM(ATM phospho S1981)水平下降。结论 miR-18a上调A549细胞放疗敏感性,其机制可能与miR-18a下调ATM有关。
[关键词] 肺癌;放射治疗;微小RNA;放射线耐受;
[中图法分类号] [文献标志码] A
Research on miR-18a enhancing radiosensitivity of A549 cells and its molecular mechanisms
Wu Lei1, Sun Jianguo1, Xu Rui1, Li Dezhi1, Xu Yanmei1, Ma Hu2, Li Xuetao1, Peng Lina1, Chen Zhengtang1 (Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Chongqing, 4000371; Cancer Hospital, Affiliated Hospital of Zunyi Medical College, Guizhou Zunyi, 5630032)
[Abstract] Objective To investigate whether miR-18a related with radiotherapy and the molecular mechanisms underlying the relationship in A549 cells. Methods After A549 cells were treated with 2 Gy X-ray, miR-18a was determined by qRT-PCR at certain times. And then after upregulating miR-18a, the radiosensitivity of A549 cells was assessed by clonogenic assay. The protein lever of Ataxia Telangiectasia Mutated (ATM) Kinase and ATM(phospho S1981) was determined by western blotting after upregulating miR-18a. Results miR-18a was decreased from ± to ±~± in A549 cells treated with 2 Gy X-ray, and miR-18a can increase radiosensitivity in A549 cells. Moreover, in A549 cells, ATM and ATM(phospho S1981) expression were downregulated by miR-18a. Conclusion In A549 cells, miR-18a can enhances radiosensitivity by inhibiting ATM and ATM(phospho S1981).
[Key words] lung neoplasms; r

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