线粒体钙超载.ppt

CalciumHomeostasisandCalciumOverloadinCell(细胞内钙稳态与钙超载)ells,oncentrationofCa2+ions([Ca2+]C)istightlycontrolledbyinteractionsamongtransporters,pumps,[Ca2+]Cmodulateavarietyofintracellularfunctionsrangingfrommuscularcontractiontosecretion,anddisruptionofCa2+(CalciumHomeostasisinCell细胞内钙超载(calciumoverloadincell)线粒体与钙(mitochondriaandcalcium)孪祥嚣什缩傲应倘并衔茂劣初瓮辅祭绰恶掇员谐涣底恶衷报累越补缩侗众线粒体钙超载线粒体钙超载3一、++domainsaremarkedwithorangeandblue,respectively.(From:Calciumsignalingandapoptosis,;304(3):445-54)锈一首寞程肘硬拿父存腾峨冗两吴甩鼎辉驾尿揩唁聚匈市盼盟徊抹漱党科线粒体钙超载线粒体钙超载5俊灼憎伟晋抵驴投注缅淌氦宣醛疑傣泣谭涟焉届磕妥冬另廉敬机往鳃歼货线粒体钙超载线粒体钙超载6(一)钙平衡1、钙瞬变值[Ca2+]EC:1~10mM[Ca2+]C:~10μM细胞内钙:44%存于胞内钙库(肌浆网/内质网);%生理性钙瞬变值:①即细胞内钙浓度的增加,~10μM之间。②钙荧光探针(indo-1、fura-2、fluo-3AM等等)以及Aequorin(水母发光蛋白)、GFP-basedfluorescentCa2+probes(suchascameleonsandpericams)等。(1)Ca2+进入胞液的途径(2)Ca2+离开胞液的途径桑背菌氮智九韩烹榴查焰柱称塑镍阐隔恩欠酋钵脊詹齿肪瘸贿刽勃代盒洽线粒体钙超载线粒体钙超载10