牙源性囊肿上皮增殖活性的研究.pdf

华西口腔医学杂志年月第卷第期
· 144 · WCJS 2001 6 19 3
牙源性囊肿上皮增殖活性的研究
孙明磊廖小宜王炼
摘要目的:研究牙源性角化囊肿、含牙囊肿、根尖囊肿 3 种主要的牙源性囊肿衬里上皮的细胞增殖活性。方法:
应用 Ki-67 单克隆抗体免疫组化 LSAB 法对 30 例牙源性囊肿进行免疫组化染色,结果通过计算机图像分析,计算单
位面积衬里上皮内(mm2)阳性细胞数,进行统计学分析。结果:牙源性角化囊肿衬里上皮有较多的 Ki-67 阳性细胞,
明显高于含牙囊肿和根尖囊肿;正常口腔粘膜未见 Ki-67 阳性表达。结论:Ki-67 在不同的牙源性囊肿中表达的差
异显示了它们具有不同的增殖和分化过程。
关键词牙源性囊肿牙源性角化囊肿 Ki-67 增殖活性
Cell Proliferation in Odontogenic Jaw Cyst Epithelium
Sun Minglei,Liao Xiaoyi,Wang Lian
The College of Stomatology,West China Uniuersity of Medical Sciences
Abstract
Objective:The aim of the study is to investigate the epithelial cell proliferation in the lining of odontogenic cysts with Ki-67
monoclonal antibody. Methods:Ki-67 expression was studied in 30 odontogenic cysts(odontogenic keratocysts,n = 10;dentiger-
ous cyst,n = 10;radicular cysts,n = 10),and normal oral epithelium(n = 10)by using a biotin streptavidin method on routinely
processed paraffin sections after microwave treatment. Ki-67 + cells were counted manually and related to the area of epithelial
lining as determined puter image analyzer. The result was analyzed by using puter image analyzer to calculate positive
cells per sguare millimeter of the epithelial lining of odontogenic lesions. Data were analyzed by using student test. Results:The
epithelial lining of odontogenic keratocysts contained the highest number of Ki-67 + cells,most of