厌氧微生物菌种保藏流程.docx

厌氧微生物菌种保藏流程厌氧微生物菌种保藏技术规程起草单位:中国农业科学院农业资源与农业区划研究所目次前言..................................................................................IV引言...................................................................................V厌氧微生物菌种保藏技术规程.............................................................61范围.................................................................................62规范性引用文件.......................................................................63术语和定义...........................................................................64保藏方法.............................................................................6附录1定期移植法.......................................................................71原理.................................................................................72技术要求.............................................................................73方法与步骤...........................................................................7附录2真空冷冻干燥保藏技术.............................................................91冷冻干燥管的制备.....................................................................92保护剂的选择和准备...................................................................93冻干样品的准备.......................................................................94预冻.................................................................................95冷冻干燥.............................................................................96真空封口及真空检验...................................................................97保藏.................................................................................98质量检查.............................................................................99保藏周期.............................................................................910复苏方法...........................................................................10附录3液氮超低温保藏技术..............................................................101安瓿管或冻存管的准备................................................................102保护剂的准备..