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头发中抽提DNA.ppt

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头发中抽提DNA并用PCR-(由简单到复杂)本科生---硕士生---博士生学****研究---创新新发现、新方法、新结论Homeworkmgkosff2005/5/8IndexPart1reviewoftwopapersPart2discussionofmyown(emphasis)Part3someinformationPart4supplementPart1ReviewoftwopapersIdentificationofanovelvariantCYP2C9alleleinChinesePart1ReviewoftwopapersBackgroundinformation:CytochromeP4502C9(CYP2C9),amemberoftheCYP2Cenzymesubfamily,(SNP)ofCYP2C9:wild-typeproteinCYP2C9*1 CYP2C9*2(Arg144Cys),CYP2C9*3(Ile359-Leu),CYP2C9*4(Ile359Thr),CYP2C9*5(Asp360Glu),CYP2C9*6(nullallele)and5’-YP2C9activityPart1ReviewoftwopapersMethodsandresults:ics(Theproceduresisomitted) ThePMstudysubject’splasmahalf-lifeisabout9–15timesthatoftheCYP2C9*1/*1orCYP2C9*1/*3carriersandisevenslowerthantheCYP2C9*3/*(CYP2C9genewasamplifiedbyPCR)andGenotypingtests(PCR–RFLPanalysis) RevealingthestudysubjectisheterozygousforCYP2C9* (PCR–RFLPanalysis---TGG)suchthatEcoRIIdigestedthePCRproductsoftheCYP2C9T269Calleleintofragmentsof192bp,195bpand302bp,whereasthePCRproductsoftheCYP2C9*1alleleweredigestedintofragmentsof387bpand302bp) %(95%%)(CYP2C9*3ANDCYP2C9*13)INTHEMETABOLISMOFLORNOXICAMINHUMANSPart1ReviewoftwopapersBackgroundinformation:CYP2C9isanimportantmemberofthecytochromeP450enzymesuperfamilywithsome12CYP2C9alleles(*1-*12),.

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