A 色谱柱和色谱系统的故障.ppt

A_色谱柱和色谱系统的故障olumnandSystemTroubleshooting色谱柱和色谱系统的故障检修WhatEveryHPLCUserShouldKnow每个HPLC使用者应该知道什么请访问中国色谱网(ponentsHPLC的组成Pump泵Injector/Autosampler进样器/自动进样器Column色谱柱Detector检测器DataSystem/Integrator数据系统/。压力问题ColumnObservationsPotentialProblems色谱柱观测潜在的问题HighpressurePluggedfrit压力高堵塞滤头Columncontamination色谱柱污染Piuggedpacking堵塞填料赣持扼麻像昨勉需朴撬乒身哮疤厂乞组岸赛段摹腐钥讲疏修蟹机院隙隐虽A_色谱柱和色谱系统的故障A_色谱柱和色谱系统的故障Date4DeterminingtheCauseandCorrectingHighBackPressure查出原因纠正压力Checkpressurewith/withoutcolumn-uard有/无柱子时检查压力-许多压力问题是由于系统或保护柱的阻塞IfColumnPressureishigh如果柱压高:Washcolumn-Eliminatecolumncontaminationand冲洗柱子pluggedpacking排除柱污染和填料堵塞-highmolecularweight/pounds高分子量/被吸附化合物-precipitatefromsampleorbuffer样品或缓冲液中的沉淀物Backflushcolumn-Clearpluggedfrit反冲柱子-清洁阻塞的滤头Changefrit-Clearpluggedfrit更换滤头猾者讨敦揩终餐蕴蕊锌忽叉钮嚼拂尚轿译窟猫榷衡讳由纠散妨旅膊颠诡捞A_色谱柱和色谱系统的故障A_色谱柱和色谱系统的故障Date5ColumnCleaning柱子清洗Flushwithstrongersolventsthanyourmobilephase用比你的流动相更强的溶剂冲洗Reversed-PhaseSolventChoicesinOrderofIncreasingStrength为了增加强度选择反相溶剂Useatleast25mlofeachsolventforanalyticalcolumns对于分析柱每种溶剂至少用25ml冲洗Mobilephasewithoutbuffersalts没有缓冲盐的流动相100%Methanol甲醇100%Acetonitrile乙***75%Acetonitrile:25%Isopropanol75乙***:25%异丙醇100%Isopropanol异丙醇100%MethyleneChloride★二***甲烷100%Hexane★已烷★WhenusingeitherHexaneorMethyleneChloridethecolumnmustbeflushedwithIsopropanolbeforereturningtoyourresvered-***甲烷柱子时,必须先用异丙醇冲洗,然后再用你的反相流动相盟怪谦铲惧亭擅骂遏嘴悔近毙痛彭殃徐革每逊横仕壬歪徒牧阎厄垂农框吭A_色谱柱和色谱系统的故障A_色谱柱和色谱系统的故障Date6ColumnCleaning柱子清洗NormalPhaseSolventChoicesinOrderofIncreasingStrength为了增加强度选用正相溶剂Useatleast50mlofeachsolvent每种溶剂至少用50ml50%Methanol:50%Chloroform50%甲醇:50%***仿100%EthylAcetate乙酸乙酯愈革钝筋叉勤煮举膝露枷崭陕痰湘移华凄隔奴轴华滓臃曾伎堪蛊胞冯蝇本A_色谱柱和色谱系统的故障A_色谱柱和色谱系统的故障Date7HowtoChangeaFritpressionFerrule压缩金属套圈Weargloves戴手套Donotallowbedtodry不要让底