阿霉素对人乳腺癌细胞MCF7表达KIF4A的抑制作用.doc

阿霉素对人乳腺癌细胞MCF7表达KIF4A的抑制作用
(作者:___________单位: ___________邮编: ___________)
作者:王露, 武慧娟, 徐宁, 王丛阳, 何泽, 沈传陆
【摘要】目的:探讨阿霉素对人乳腺癌细胞MCF7表达KIF4A的影响。方法:用Western blotting方法检测阿霉素处理MCF7细胞后不同时间该细胞表达的KIF4A蛋白;用不同浓度的阿霉素处理MCF7细胞96 h后,检测KIF4A蛋白,并进行衰老相关半乳糖苷酶染色。结果:与未用阿霉素处理的MCF7细胞相比,阿霉素处理的MCF7细胞表达KIF4A蛋白降低;衰老相关半乳糖苷酶染色率由5%提高到42%,染色阳性的细胞形态亦呈衰老样变。结论:阿霉素处理MCF7细胞可使KIF4A蛋白表达水平降低,KIF4A蛋白表达降低与阿霉素引起MCF7细胞衰老的关系有待进一步研究。
【关键词】 KIF4A蛋白; 阿霉素; 细胞衰老; MCF7细胞
[Abstract] Objective: To study the effects of adriamycin on expression of KIF4A protein in MCF7 cells. Methods: During different periods of time after the cells treated with adriamycin, the expression level of KIF4A protein was detected by Western blotting. Then, 96 h after the MCF
7 cells were treated with different concentrations of adriamycin, the expression of KIF4A protein was monitored by Western blotting, and at the same time the senescent MCF7 cells were measured by using the method of senescence associatedβgalactosidase stain. Results: Compared with the untreated MCF7 cells, the expression level of KIF4A protein in the MCF7 cells treated with adriamycin was significantly decreased. The MCF7cells positive for the senescence associatedβgalactosidase marker were increased from 5% to 42% by 1 μmol·L-1 of adriamycin. Meantime, the positive staining MCF7 cells exhibited a characteristic enlarged and flattened morphology. Conclusion: