阿司匹林对人γδT细胞杀伤消化系统肿瘤细胞的影响.doc

阿司匹林对人γδT细胞杀伤消化系统肿瘤细胞的影响
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作者:刘军权, 陈复兴, 朱斌, 张颂张娟, 陶征中, 李佚
【摘要】目的: 探讨阿司匹林对人γδT细胞杀伤消化道肿瘤细胞株的影响。方法: 用异戊烯焦磷酸法体外扩增人外周血γδT细胞。用不同浓度的阿司匹林诱导γδT细胞和消化道肿瘤SGC7901、 SW1990、 SW480、 SW1116、 LOVO细胞株, 用乳酸脱氢酶法测定γδT细胞的杀伤活性, 用流式细胞术(FCM)检测阿司匹林诱导前后的γδT细胞和SGC7901、 SW1990、 SW480、 SW1116和LOVO细胞凋亡百分率。结果: γδT细胞培养10 %%。~ mmol/L诱导24 h后的γδT细胞对5种肿瘤细胞的杀伤活性最高, mmol/L时杀伤活性呈下降趋势; SGC7901、 SW1990、 SW480、 SW1116和LOVO细胞经不同浓度的阿司匹林诱导24 mmol/L时γδT细胞对SW480、 SW1116和LOVO细胞株的杀伤活性略有增强外, 其他组与对照组比较无明显变化。 mmol/L阿司匹林对
γδT细胞诱导24 h的凋亡率(%)明显高于SGC7901、 SW1990、 SW480、 SW1116和LOVO细胞株(%、 %、 %、 %和%)。结论: 阿司匹林在临床常规使用的药物浓度时可增强γδT细胞杀伤肿瘤细胞作用, 超过这一浓度可明显抑制γδT细胞的增殖能力和杀伤活性及增加γδT细胞的凋亡率, 而对SGC7901、 SW1990、 SW480、 SW1116和LOVO细胞株作用不明显。
【关键词】γδT细胞; 阿司匹林; 消化系统肿瘤细胞株
[Abstract] AIM: To explore the effect of aspirin on human’s γδT cells killing digestive system tumor cell lines. METHODS: Use the isopentenyl pyrophosphate method to amplify human peripheral blood γδT cells in vitro. Various concentrations of aspirin were used to induce γδT cells and digestive system tumor cells SGC7901, SW1990, SW480, SW1116, LOVO cells lines, LDH assays was used to measure the cytotoxic activity of γδT cells, flow cytometry analysis the apoptosis percentage of before and after induced γδT cells and SGC7901, SW1990, SW480, SW1116, LOVO cell lines. RESULTS: γδT cells were cultivated for ten days which proliferation ratio increase from % to %. When γδT cells induced via aspirin concentrations rang from mmol/L to mmol/L which cytotoxic activity on the five kinds of tumor cell lines was the highest, if aspirin
’s concentration surpassing mmol/L, cytotoxic activity of γδT cells present decrease tendency. SGC7901, SW1990, SW480, SW1116, LOVO cells lines were induced by different concentrations of aspirin for 24 hours, just SW480, SW1116 and LOVO were enhanced as far as the cytoto