分子生物学课件.pptMolecularBiologyCourseSectionK:TranscriptioninprokaryotesDNARNAPROTEINtoday’,RNA合成的过程(起始,延伸,终止)功能,a亚基,b亚基,b’亚基,sigma(s)因子启动子,s70大小,-10序列,-35序列,转录起始位点,启动子效率启动子结合,解旋,RNA链起始,延伸,终止(r蛋白因子)SectionK:TranscriptioninprokaryotesMolecularBiologyCourseK1:转录基本的原则Transcription:综述(相对复制而言)TheprocessofRNAsynthesis:initiation,elongation,terminationMolecularBiologyCourseK1-1:Transcription:anoverviewKeytermsdefinedinthissection(GeneVII)CodingstrandofDNAhasthesamesequenceasmRNA.Downstreamidentifiessequencesproceedingfartherinthedirectionofexpression;forexample,+1upstreamdownstreamUpstreamidentifiessequencesproceedingintheoppositedirectionfromexpression;forexample,thebacterialpromoterisupstreamfromthetranscriptionunit,;,representedattheendofthetranscript,(formallydescribedasDNA-dependentRNApolymerases).:2DNAmoleculesTranscription::parisonwithreplicationSynthesisoftheOkazakifragmentsOppositetothereplicationforkmovementSynthesisoftheleadingstrandReplicationinthesamedirectionasthereplicationforkmoves
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