英文-学术报告.ppt

CharacteristicsofEscherichiacolibiofilmproduction,ictyping,drugresistancepatternandgeneexpressionunderaminoglycosidepressureserichiacoli大肠杆菌infection感染Aminoglycoside氨基糖苷类antibiotic抗生素Pseudomonas假单胞菌aeruginosa聚合酶链反应技术Crystalvioletstainingmethod结晶紫染色法phenotype表现型drugresistancepattern耐药模式Antimicrobialagent抗菌药物molecularbiologyreagent分子生物学试剂Brothmicrodilutionmethod肉汤稀释法Susceptibilitytesting药敏试验Genotype基因型Real-timeqRT-PCR实时定量荧光PCRStreptomycin链霉素amikacin丁***,groundSeveraltypesofinfectioncausedbyEscherichiacoli(.,urethralcatheter,biliarytractprosthesis,biliarytractinfection,lithangiuria,urinarytractinfectionandmanyotherdiseases)areassociatedwithbiofilmformation,aturetoresisthighlevelsofantibiotics(FontaineandSmith,2006;Melchioretal.,2006)..groundInaddition,underextensiveandpersistentpressureofantibiotics,inordertosurvive,.(2005)(Navesetal.,2008;Rijavecetal.,2008).ractInthisstudy,qualitative(scanningelectronmicroscope)andsemi-quantitative(modifiedcrystalvioletstainingmethod)methodshadbeenusedtoevaluateEscherichiacolibiofilm--basedPCR(ERIC-PCR),westudiedthecorrelationbetweenbiofilm-formingabilityphenotype,-timeqPCR(qRT-PCR)(acrA,agn43,csgA,csgD,ompFandpgaA):(i)-formingabilitybasedonthequalitative(scanningelectronmicroscope)andquantitative(modifiedcrystalvioletstainingmethod)methods.(ii)Thecorrelations