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系列抗心肌多肽自身抗体对扩张型心肌病的诊断价值.doc
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系列抗心肌多肽自身抗体对扩张型 心肌病的诊断价值        摘要目的:探讨抗人工合成多肽自身抗体在扩张型心肌病(DCM)诊断中的价值。方法:根据计算机预测心肌ADP/ATP载体(ANT)、β1受体、M2受体和肌球蛋白重链(MHC)抗原决定簇的氨基酸序列并合成多肽片段,以此片段作为抗原,同时用天然抗原对比,以酶联免疫吸附方法检测48例DCM患者(DCM组)和48例健康献血员(对照组)血清中抗心肌多肽自身抗体。结果:DCM组中抗ANT抗体、抗β1受体抗体、抗M2受体抗体和抗MHC抗体阳性分别有31、26、20和23例,与对照组比较有显著性差异(P<0.005),且与天然心肌抗原检测结果高度一致(P<0.01)。结论:人工合成多肽可代替天然心肌抗原用于检测抗心肌自身抗体,抗心肌多肽抗体检测可以作为DCM免疫学诊断方法。关键词心肌病,充血性抗心肌多肽自身抗体DiagnosticvalueofserialautoantibodiesagainstcardiacpeptidesindilatedcardiomyopathyYUANHai-tao LIAOYu-hua WANGMin DONGJi-hua CAOLin-shengWANGZhao-hui WANGJin-ping LUYin-ping(InstituteofCardiology,TongjiMedicalUniversity,Wuhan430022) Abstract Objective:Toinvestigatethediagnosticvalueofautoantibodiesagainstcardiacpeptidesindilatedcardiomyopathy(DCM)byusingantigensofsyntheticpeptidesintheexamination.Method:puterprediction,wesynthesizedpeptideswhoseaminoacidsequenceswerederivedfromdeterminantsofcardiacantigensincludingtheadeninenucleotidetranslocator(ANT),β1-adrenoceptor,M2muscarinicreceptorandmyosinheavychain(MHC).Takingthissyntheticpeptidesasantigens,autoantibodieswereexaminedbyanELISAmethodin48patientswithDCMand48healthydonors,paredtheresultsbyusingnaturalcardiacantigens.Result:Of48serafrompatientswithDCM,31(64.6%)boundtothepeptidesderivedfromANT,23(47.9%)reactedwiththesequencefrommyosinheavychain,26(54.2%)and20(41.7%)werepositiveinreactingwithpeptidesfromβ1-adrenoceptorandM1muscarinicreceptorrespectively.Theseresultsshowedsignificantdifferencebetweenthesetwogroups.Sotheantigenicityoftheselecteddeterminantswasconfirmed,moreover,theresultswereapproximatellyconsistentwiththatofnaturalantigen.Conclusion:Syntheticpeptidesderivedfromdeterminantsofcardiacantigenscansubstitutefornaturalantigenstoscreenautoantibodies.TheexaminationofautoantibodiesagainstcardiacpeptidescanbetakenasthemethodofimmunologicaldiagnosisofDCM. 内容来自淘豆网www.taodocs.com转载请标明出处.