心肌缺血预处理对未成熟心肌细胞凋亡影响.doc

心肌缺血预处理对未成熟心肌细胞凋亡影响                  作者:孙忠东,池一凡,杨铁南,侯文明,牛兆倬,唐子龙    【关键词】 缺血预处理   摘要:目的 探讨心肌缺血预处理(MIP)对未成熟心肌bcl-2、bax、fas蛋白表达的影响。方法采用兔Langendorff模型,24只幼兔随机分为3组,对照组(C,n=8):仅灌注KH液180min,然后取标本;缺血/再灌注组(I/R,n=8):灌注20min后,停灌45min,复灌120min;心肌缺血预处理组(MIP,n=8),灌注20min,反复2次停灌5min,复灌5min,然后重复I/R组缺血/再灌方法。以凋亡细胞原位标记、琼脂糖凝胶电泳检测DN***段梯及bcl-2、bax、fas蛋白表达作为检查指标。结果琼脂糖凝胶电泳检测DN***段梯:MIP组与I/R组比较,光密度明显减弱;MIP组与I/R组比较,bcl-2表达明显增多,bax、fas表达明显减少。结论MIP通过影响bcl-2、bax、fas基因蛋白的表达减少心肌细胞凋亡。关键词:缺血预处理;心肌;细胞凋亡;基因蛋白 TheEffectsofMyocardialIschemicPreconditioningonImmatureMyocardialCellApoptosis     Abstract:OBJECTIVEToinvestigatetheeffectsofmyocardialischemicpreconditioning(MIP)onimmaturemyocardialcellapoptosisand expressionofbcl-2,bax,,24rabbits(aged14~21days)weredividedinto3groups(n=8).ControlgroupwasonlyperfusedwithKrebs-Henseleit(KH)(I/R)groupwasperfused20minutes,andthenkeptunderischemia45minutes,(MIP)groupwasperfused20minutes,andthenrepeatlyischemia5minutesfollowedreperfusion5minutes2times,,includingtheTUNEL,nucleosomalladderofDNAfragments,andtheexpressionofbcl-2,bax, MIPgrouptherewasreductioninTUNELcellapoptosis,ladderofDNAfragments,expressionofbaxandfasgene,andincreaseinexpressionofbcl