促红细胞生成素增加k562细胞转铁蛋白受体的表达及其对细胞周期的影响.doc

促红细胞生成素增加K562细胞转铁蛋白受体的表达及其对细胞周期的影响?528中华儿科杂志2003年7月第41卷第7期ChinJPediatr,July2003,Vol41,?论著?促红细胞生成素增加K562细胞转铁蛋白受体的表达及其对细胞周期的影响周密廖清奎李丰益高举符仁义罗春华李强贾苍松【摘要】binanthumanerythroipoitin,rhEPO和铁对白血病细胞K562转铁蛋白受体transferrinreceptor,TfR即CD抗原表达的影响,及其相同处理因素对71K562细胞周期变化的影响。方法体外培养K562细胞并加rhEPO等处理因素,分别在24h和72h终止处理,各处理组细胞分别与FITC荧光标记的CD单抗孵育,或与DNA染料碘化丙啶作用。通过71流式细胞分析技术检测CD抗原的表达和细胞周期,观察各组TfR表达情况和S期细胞百分率。71结果1不同浓度的rhEPO培养K562细胞72h,均可使TfR表达增加,与同期空白对照组相比差异有显著意义P0105。2单独使用rhEPO5UPml或rhEPO5UPml+FeCl100μmolPL培养细胞723h可使S期细胞百分率上升,且与空白对照组相比差异有显著意义P0105。结论rhEPO可通过增加白血病细胞转铁蛋白受体的表达而增加DNA的合成。【关键词】红细胞生成素;铁;受体,转铁蛋白;铁蛋白;K562细胞;ellcycleZHOUMi,LIAOQing2kui,LIFeng2yi,GAOJu,FURen2yi,LUOChun2hua,LIQiang,JIACang2song1DepartmentofPediatrics,TheSecondHuaxiHospital,SichuanUniversity,Chengdu610041,China【Abstract】ObjectiveFunctionally,erythropoietinEPOcanpromotetheproliferationandgrowthoferythroidprogenitorcells,anditiswidelyusedinthetreatmentofanemiainchronicdiseasescausedbytumorandinflammation1However,binanthumanEPOrhEPOontheexpressionoftransferrinreceptorTfR,ellK562anditsrelationtocellcycle171MethodsInvitrocultureofK562cellwasperformedwithadditionsofvariousconcentrationsofrhEPOandFe1Treatmentswereterminatedat24hand72h,respectively1TheneachgroupofcellswasincubatedwithFITC2IgGantibodytoCDorPI,akindofDNAdye1AndTfRexpressionandDNAsynthesisstatuswereanalyzedbyflow271cytometry1Results1TheexpressionofTfRbyK562cellsincreasedsign