一种用于二维凝胶电泳的微流控芯片.doc


一种用于二维凝胶电泳的微流控芯片#
陈宏*
(厦门大学微机电中心,厦门 361005)
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摘要:二维凝胶电泳是常用的蛋白质分离方法,但其有耗时费力等不足。这里在微流控芯片
上进行二维电泳的方法,在两维通道的界面上沉积一层二氧化钛薄膜。这层二氧化钛薄膜用
于加强两维之间的结构,间隔两维间的不同的缓冲液体系,并且防止等电聚焦过程中蛋白质
扩散进入第二维。文章分别对等电聚焦和凝胶电泳过程分别进行了考察,并在最后进行了差
异二维电泳的尝试。
关键词:微流控芯片;二维电泳;接口;蛋白质
中图分类号:
A Microfluidic Chip Used for the Two-Dimensional
Electrophoresis
Hong Chen
(MEMS Research Center, Xiamen University, Xiamen 361005)
Abstract: Two-dimensional is a very useful method in the protein analysis, but the procedure is
labor intensive and time consuming. Here, two dimensional was performed on a microfluidic chip,
a membrane of titanium dioxide was deposit at the interface of two dimensionals to enhance the
performance of separation. The titanium dioxide membrane separated the different buffers for the
integrating isoelectric focusing (IEF) and polyacrylamide gel electrophoresis (PAGE). It also
prevented the protein from diffusing into the second dimension during the IEF. After IEF, the
focused proteins were then transferred into the second dimension under the electric field. The
process of IEF and PAGE was studied and differential gel electrophoresis was performed to test its
performance.
Key words: Microfluidic chip; Two-dimensional electrophoresis; Interface; Protein
0 引言
二维凝胶电泳生物实验室中广泛应用的一种分离方法,经常用于蛋白质分离,其中第一
维是等电聚焦,第二维是聚丙烯酰胺凝胶电泳。二维凝胶电泳在一块凝胶上能分离并检测几
千甚至上万种蛋