鬼臼毒衍生物诱导人肺腺癌a549细胞凋亡鬼臼毒衍生物诱导人肺腺癌A549细胞凋亡【关键词】 鬼臼***,肺肿瘤,腺癌,,细胞凋亡,抗原,CD95,基因,BclApoptosisinhumanlungadenocarcinomacelllineA549byderivativeof podophyllotoxin【Abstract】 AIM: Tostudytheapoptosisofhumanlung 2,aderivativeofpodophyllotoxin,: MTTassay,electronmicroscopy,,,48,,,,;agarosegelelectrophoresisshowedanevidentDNAfragmentation;cellcycleanalysisindicatedanincreasedSphaseproportion,aswellasanapparentS/2decreasedBcl2proteinexpression(P<):&2couldinhibitthegrowthofA549cellsviatheinductionofS/G2phasearrest,whichisprobablyassociatedwiththedecreasedBcl2expressionandcellcyclearrest.【Keywords】 podophyllotoxin;lung neoplasms;adenocarcinoma;apoptosis;antigens,CD95;genes,Bcl2【摘要】 目的: 2对人肺腺癌细胞A549的凋亡诱导作用~: 采用四***偶氮唑蓝(MTT)比色法、电子显微镜、DNA琼脂糖凝胶电泳和细胞周期分析法检测A549细胞凋亡,采用流式细胞术(FCM)测定细胞Bcl2,Fas及caspase3蛋白表达水平. 结果:&2对A549细胞增殖具有明显的剂量和时间依赖的抑
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