浅论食管癌患者外周血CD4 CD25 Foxp3 调节性T细胞检测及其临床意义.doc

浅论食管癌患者外周血CD4+CD25+Foxp3+调节性T细胞检测及其临床意义                作者:付白清,吴朝阳,许亚平,王法春,陈巧云【摘要】  目的:通过检测食管癌患者外周血中CD4+CD25+Foxp3+调节性T细胞的变化,观察其与临床及病理特征之间的关系,探讨其临床意义。方法:采用流式细胞术检测30例食管癌患者及16例健康志愿者外周血单个核细胞中CD4+CD25+Foxp3+T细胞占CD4+T细胞的比例;收集食管癌患者的临床及病理资料,分析研究Treg细胞变化与临床及病理特征之间的关系。结果:流式细胞术检测显示食管癌患者外周血PBMC中CD4+CD25+Foxp3+T细胞占CD4+T细胞的比例为(±)%,高于对照组(±)%,;食癌管患者外周血中Treg细胞比例与患者的年龄、性别、肿瘤的位置无明显相关性(),而与淋巴结转移情况及TNM分期有明显的相关性()。结论:CD4+CD25+Foxp3+调节性T细胞可能在食管癌免疫耐受中发挥重要作用,检测其比例对于判断食管癌的病期及预后有一定价值。【关键词】 调节性T细胞;食管癌;流式细胞术[Abstract]Objective:TofindoutthechangeofCD4+CD25+Foxp3+regulatoryTcellsinperipheralbloodofesophagealcancerpatients,therelationshipbetweenclinicaldataandpcorholozicalcharacteristics,:FlowcytometrywasusedtoanalyzetheproportionofCD4+CD25+Foxp3+Tcells/CD4+Tcellsinperipheralbloodmononuclearcells(PBMCs),andttestwasusedtoanalyzetherelationshipbetweentheproportionofTregcellsinPBMCandclinicaldataandpathologicalcharacteristics. Results:TheproportionofCD4+CD25+Foxp3+Tcells/CD4+TcellsinPBMCfromesophagealcancerpatientswassignificantlyhigherthanthatofcontrols().TheproportionofTregcellsinPBMChasnosignificantdifferencewithsex,age,locationoftumor,categoryofpathologyandtheinfiltratingdepthoftumor().TheproportionofT