浅论高氧液对家兔心肌缺血再灌注损伤的保护作用及与ICAM-1的关系.doc

浅论高氧液对家兔心肌缺血再灌注损伤的保护作用及与ICAM-1的关系              作者:王德明,刘艳阳,桂靖,常业恬,刘梅梅【摘要】 目的:研究高氧液对家兔心肌缺血再灌注损伤的保护作用与细胞间黏附分子(ICAM-1)的关系。方法:56只家兔随机分为3组:假手术对照组(P组)、缺血再灌注损伤组(IR组)、高氧液灌注组(HBO组)。分别取缺血危险区心肌,免疫组化法检测ICAM?的表达;检测匀浆中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;计算心肌梗死面积。结果:HBO组、IR组SOD活性均较P组降低();MDA含量则较P组增加()。HBO组心肌梗死面积较IR组明显减小()。ICAM-1的表达,IR组比HBO、P组都明显增高()。结论:高氧液处理可下调心肌缺血再灌注损伤时ICAM-1的表达,使SOD活性升高,从而降低心肌梗死面积。【关键词】 高氧液;心肌梗死面积;超氧化物歧化物;丙二醛;细胞间黏附分子  Abstract: Objective:TostudytheeffectsofhyperbaricoxygenanditsrelationshipwithICAM-:Fifty-sixfamilyrabbitswereallocatedtocontrolgroup,,ICAM-1weredetectedwithimmunohistochemtry,theactivityofsuperoxidedismutase(SOD)andthecontentofmalondialdehyde(MDA)inhomogenateweredetected,:TheactivityofSODwasloweringroupIRandandgroupHBOthanthatincontrol(),whilethequantityofMDAishigher().MyocardialinfarctionareawassmalleringroupHBOthanthatingroupIR().TheexpressionofICAM-1wassignificantlyup-:Hyperbaricoxygentreatmentdown-regulatestheexpressionofICAM-1andincreasestheactivityofSOD,therebyreducingtheinfarctiona