腺苷受体激动剂在豚鼠实验性哮喘中的作用.doc

腺苷受体激动剂在豚鼠实验性哮喘中的作用        [摘要] 目的:研究腺苷受体激动剂R-苯异丙基腺苷(N6-phenyl-2R-isopropyl-adenosine,R-PIA)对实验性哮喘豚鼠气道阻力、肺顺应性的影响。方法:复制豚鼠实验性哮喘动物模型,给予R-PIA5mg/mL的生理盐水雾化吸入,观察气道阻力、肺顺应性的变化,以及肺泡灌洗液中NO-x、环-磷酸腺苷(cAMP)、环一磷酸鸟苷(cGMP)、肿瘤坏死因子(TNF)含量的变化。结果:给予R-PIA雾化吸入后较哮喘组气道阻力增加(P<),肺顺应性下降(P<),肺泡灌洗液中NO-x、cAMP、cGMP、TNF水平下降,而TNF含量无明显变化。±,R-±(P<),±,R-±(P<),±,R-±(P<),±,R-±(P>)。结论:R-PIA有收缩气道的作用,并与肺泡灌洗液中NO-x、cAMP、cGMP水平下降有关。[主题词] 豚鼠;哮喘;动物,实验;苯异丙基腺苷[中分类号] +5 [文献标识码] A [文章编号]1000-4718(2000)02-0135-04EffectsofinhalingN6-phenyl-2R-isopropyl-adenosineontheexperimentalasthmaticguinea-pigsLIRong,ZHANGZhen-xiang,PENGZe(RespiratoryDiseasesResearchLaboratory,DepartmentofMedicine,TongjiHospital,TongjiMedicalUniversity,Wuhan430030,China) [Abstract]AIM:ToevaluatetheroleofinhalingN6-phenyl-2R-isopropyl-adenosine(R-PIA)plianceofexperimentalasthmaticguinea-:Experimentalmodelofasthmaticguinea--PIA5mg/mL(5mgR-%saline).Theairwayresistance,plianceandNO-x,cyclicadenosinemonophosphate(cAMP),cyclicguanosinemonophosphate(cGMP),tumornecrosisfactor(TNF)inbronchoalveolarlavagefluid(BALF),afterinhalingR-PIA,theairwayresistanceofexperimentalasthmaticguinea-pigsincreased(P<),pliancedecreased(P<).SodidtheNO-xandcAMP,cGMPinBALF (P<),TNFinBALFhadnochange (P>).CONCLUSION:InhalingR-PIAcouldconstricttheairwayofexperimentalasthmaticguinea-pigs.