产纤维素酶真菌的分离筛选、鉴定及其酶学性质分析.pdf

基因组学与应用生物学,2013年,第32卷,第3期,第372-378页Genomics and Applied Biology, 2013, , , 372-378研究报告Research Report产纤维素酶真菌的分离筛选、鉴定及其酶学性质分析宋贤冲1,2,3唐健1,2,3*邓小军1,2,3魏世清1,2,3梁艳芳41广西壮族自治区林业科学研究院,南宁, 530002; 2国家林业局中南速生材繁育实验室,南宁, 530002; 3广西优良用材林资源培育重点实验室,南宁, 530002; 4广西国有七坡林场,南宁, 530225*通讯作者, 21712104@摘 要采用梯度稀释涂布选择性平板的方法从腐烂的桉树伐桩中分离筛选出一株产纤维素酶的真菌菌株,将其编号为F01。对菌株F01的羧甲基纤维素、滤纸和结晶纤维素酶活力进行了测定, IU/mL、 IU/ IU/mL。形态和ITS序列鉴定菌株F01为木霉属真菌。系统发育分析结果表明,木霉属菌株F01和棘孢木霉T20为一个共同分枝,且进化距离较近。℃,酶活力在偏酸性条件下稳定且在30~55℃范围内具有一定的耐热性。木霉属真菌F01有较高的纤维素酶活力,其在加快桉树伐桩腐烂进程方面可能具有良好的应用前景。关键词桉树,伐桩,纤维素酶,木霉属Isolating, Screening and Identifying of Cellulase Producing FungiSong Xianchong1,2,3Tang Jian1,2,3*Deng Xiaojun1,2,3Wei Shiqing1,2,3Liang Yanfang41 Guangxi Zhuang Autonomous Region Forestry Research Institute, Nanning, 530002; 2 Key Laboratory of Central South Fast-growing TimberCultivation of Forestry Ministry of China, Nanning, 530002; 3 Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Nanning,530002; 4 Guangxi Qipo State Forest Farm, Nanning, 530225* Corresponding author, ******@DOI: .000372AbstractDecayedEucalyptusstumps was taken as the sample to screen the microbes which can accelerate thedecay of stumps. Using gradient dilution and spread selective plate methods, a fungi strain F01 was screened. TheCMCase, FPAand Avicelase of strain F01 w